betadex and propargylamine

The retention of 17 propargylamine derivatives was determined on a beta-cyclodextrin polymer (beta-CDP)-coated silica column using tetrahydrofuran-0.05 M K2HPO4 (6:4, v/v) as eluent. The inclusion complex formation between the propargylamine derivatives and a water-soluble beta-CDP was studied by charge-transfer chromatography carried out on reversed-phase TLC layers. The capacity factors were correlated with the various measured and calculated physicochemical parameters of the solutes using principal component analysis followed by non-linear mapping, varimax rotation and cluster analysis. Calculations proved that the hydrophobicity and steric parameters have the highest influence on the retention of propargylamine derivatives. It has been established that each statistical method can be used for the evaluation of similar retention data matrices, however, the results can be slightly different according to the method applied.

Topics: beta-Cyclodextrins; Chromatography, High Pressure Liquid; Chromatography, Thin Layer; Cluster Analysis; Cyclodextrins; Furans; Molecular Structure; Monoamine Oxidase Inhibitors; Multivariate Analysis; Pargyline; Propylamines; Regression Analysis

The retention of 17 monoamine oxidase inhibitory drugs (proparlgylamine derivatives) were determined on a beta-cyclodextrin polymer (beta CDP)-coated silica column using ethanol-0.05 M K2HPO4 (6:4 v/v) as the eluent. The relative strength of interaction between the drugs and a water soluble beta-cyclodextrin polymer was determined by charge-transfer chromatography carried out on reversed-phase TLC layers. The relationship between capacity factors, physicochemical parameters and inclusion complex forming capacity of the monoamine oxidase inhibitory drugs were evaluated by stepwise regression analysis and by principal component analysis (PCA) followed by two-dimensional nonlinear mapping and varimax rotation. Calculations indicated that the retention of monoamine oxidase inhibitory drugs on beta CDP column is mainly governed by their steric and lipophylic parameters. Significant linear correlations were found between the corresponding coordinates of varimax rotation and two-dimensional nonlinear maps proving the suitability of both methods for the reduction of dimensionality of complicated data matrices.

Topics: beta-Cyclodextrins; Chemical Phenomena; Chemistry, Physical; Chromatography, High Pressure Liquid; Chromatography, Thin Layer; Cyclodextrins; Food Additives; Monoamine Oxidase Inhibitors; Multivariate Analysis; Pargyline; Propylamines; Solubility