betadex has been researched along with monoisopropanolamine* in 1 studies
1 other study(ies) available for betadex and monoisopropanolamine
| Article | Year |
|---|---|
Comparative application of microwave, ultrasonication, ultracentrifugation and conventional heating for preparation of sample as dinitrophenyl derivative for direct enantioseparation of certain amino alcohols and 1-amino-2-propanol from vitamin B12 hydrol
Taking into account the structural similarities of amino alcohols with amino acids and in order to reduce time for derivatization unconventional approaches viz. microwave irradiation, ultrasonication and ultra centrifugation were applied for synthesis of dinitrophenyl derivatives of nine amino alcohols and to work out a method of choice for sample preparation for direct enantioseparation. The enantiomeric dinitrophenyl derivatives so synthesized were separated on alpha(1)-acid glycoprotein and beta-cyclodextrin columns with detection at 230nm using photodiode array detection system. Derivatization methods and chromatographic parameters were optimized. beta-Cyclodextrin column was found better compared to AGP column for enantioseparation. Limit of detection, quantification, accuracy and precision were also determined. The developed method was successfully applied to determine enantiomeric purity of 1-amino-2-propanol obtained from vitamin B(12) hydrolysate. Topics: Amino Alcohols; beta-Cyclodextrins; Chromatography; Dinitrobenzenes; Glycoproteins; Hot Temperature; Microwaves; Propanolamines; Protein Hydrolysates; Sonication; Stereoisomerism; Ultracentrifugation; Vitamin B 12 | 2009 |