betadex and hydrocortisone-acetate

  Pressure sores are lesions caused by impaired blood flow. Conventional dressings can absorb exudates, but do not promote wound healing. A hydrogel composed of β-cyclodextrin (β-CD), polyethyleneimine (PEI) and silk fibroin (SF) was assessed for use in healing of pressure sores.. The hydrogel was prepared by crosslinking β-CD-grafted PEI and SF using epichlorohydrin. The gel was then immersed in an aqueous solution of Centella asiatica extract (CAE) 0.7 mg/mL and/or hydrocortisone acetate (HCA) 0.5 mg/mL. The in vivo pressure sore-healing efficacy of the dry gel (with or without the drugs) was investigated in terms of the hyperplasia of epidermis and the number of neutrophils in the skin tissue.. The specific loading of CAE was 0.0091 g/g of dry gel. The percentage of CAE released at 24 h at pH 3.0, 5.0 and 7.4 was approximately 63.9%, 55.0% and 44.4%, respectively. This pH-dependent release is possibly due to the degree of gel swelling, which decreased with increasing pH. The specific loading of HCA was 0.0050 g/g dry gel, and the percentage release of HCA at 24 h was around 20% at all three pH points. It is likely that HCA release is independent of pH. HCA is a hydrophobic compound, and therefore the release of HCA is affected by the partitioning of HCA between the β-CD cavity and the bulk water phase, but not by the degree of swelling of the hydrogel. The pressure sores treated with the hydrogel healed in 6 days, compared with 10 days for controls.. In this study, a β-CD/PEI/SF hydrogel containing CAE and HCA reduced the healing time for pressure sores.

Topics: Animals; beta-Cyclodextrins; Centella; Fibroins; Hydrocortisone; Hydrogels; Male; Mice; Mice, Inbred ICR; Plant Extracts; Polyethyleneimine; Pressure Ulcer; Silk; Skin; Triterpenes; Wound Healing

The effect of the complexation with beta-cyclodextrin, hydroxypropyl beta-cyclodextrin and polyvinylpyrrolidone on the diffusion kinetics of hydrocortisone acetate through a non porous lipidic membrane was analyzed starting from different dermal bases: a Carbopol gel and lanovaseline. A constant diffusive gradient was achieved; this suggests that the complexation equilibrium controls the diffusable form, according to its stability constant. The following sequence was observed for the cumulative amount diffused: hydrocortisone acetate greater than hydrocortisone acetate/polyvinylpyrrolidone greater than hydrocortisone acetate/hydroxypropyl beta-cyclodextrin greater than hydrocortisone acetate/beta-cyclodextrin. Such behaviour was analyzed in terms of the main physical chemical parameters of the systems examined.

Topics: Acrylic Resins; Administration, Topical; beta-Cyclodextrins; Chemical Phenomena; Chemistry, Physical; Cyclodextrins; Diffusion; Hydrocortisone; Membranes, Artificial; Polyvinyls; Povidone; Silicone Elastomers