betadex and cuminaldehyde

E. coli O157:H7 is one of the most common food-borne pathogens and usually related to contaminated vegetables. This study was to prepare an effective antibacterial agent and applied in vegetable juices. In this study, β-cyclodextrin inclusion complexof CUM (CUM /βCD-IC) was prepared using ultrasonication technique and then treated with cold nitrogen plasma (CNP) to observe its effect in the physicochemical and antibacterial properties of CUM/βCD-IC. Various characterization techniques such as fluorescence, fourier transform infrared spectroscopy (FT-IR), thermal gravimetric analysis (TGA), differential scanning calorimetry (DSC) and X-ray diffraction (XRD) confirmed the formation of IC between CUM and βCD. Phase solubility and double reciprocal profiles studies proved the enhanced solubility of CUM with increasing amount of βCD and the guest/host stoichiometry of 1:1. Computational modeling and FT-IR indicated that the phenyl ring with isopropyl chain of CUM is inserted in the hydrophobic βCD. Investigations of thermal properties proved that the βCD-IC formation improved the stability of CUM. Antibacterial test results indicated that CNP-CUM/βCD-IC exhibited better antibacterial activity than CUM/βCD-IC. After CNP-CUM/βCD-IC treatment, it was observed by TEM that the cell membrane of E. coli O157:H7 was broken. In addition, the antibacterial activity of CNP-CUM/βCD-IC in vegetable juices was carried out and the findings revealed that CNP-CUM/βCD-IC has an excellent antibacterial effect on vegetable juices.

Topics: Benzaldehydes; beta-Cyclodextrins; Cymenes; Escherichia coli; Fruit and Vegetable Juices; Nitrogen; Plasma Gases; Spectroscopy, Fourier Transform Infrared

Polysaccharide substrates loaded with antioxidant and antimicrobial compounds, effectively protected by cyclodextrin moieties, can be a long-lasting solution to confer certain properties to fabrics, paper and other materials. β-Cyclodextrin was attached to α-cellulose, bleached pulp and starch by a two-step esterification with a tetracarboxylic acid. The resulting derivatives were characterized by spectroscopy, thermal degradation analysis and capability of phenolphthalein inclusion. The carriers, containing between 89 and 171 μmol of β-cyclodextrin per gram, were loaded with carvacrol, cuminaldehyde, cinnamaldehyde and hydroxytyrosol. From a stoichiometric addition, the percentage of compound retained ranged from 49% (hydroxytyrosol in pulp-cyclodextrin) to 92% (carvacrol in starch-cyclodextrin). Finally, the release rate to aqueous ethanol was measured over eight days and fitted to kinetic models. From the analysis of the mean dissolution time, it can be concluded that inserting β-cyclodextrin units enhanced the long-term holding of phenolic active compounds in carbohydrate matrices.

Topics: Acrolein; Antioxidants; Benzaldehydes; beta-Cyclodextrins; Butanes; Carboxylic Acids; Cellulose; Cross-Linking Reagents; Cymenes; Drug Liberation; Kinetics; Phenols; Phenylethyl Alcohol; Starch