betadex and bioallethrin

A capillary micellar electrokinetic chromatography (MEKC) method was developed enabling the stereoselective separation of the insecticide bioallethrin. The use of sodium deoxycholate bile salt and acetyl-β-cyclodextrin (acetyl-β-CD) made possible the separation of bioallethrin stereoisomers with a high enantioresolution (7.4) in a short analysis time (6.5min). The analytical characteristics of the developed method were evaluated in terms of linearity, accuracy, precision, and limits of detection (LOD) and quantitation (LOQ) showing a good performance for the quantitation of bioallethrin stereoisomers with LODs of 0.2 and 0.3mg/L. The developed method was applied to the stereoselective analysis of a commercial bioallethrin pediculicide formulation and to evaluate the toxicity of bioallethrin stereoisomers on the growth of the unicellular freshwater green alga Pseudokirchneriella subcapitata and on the germination of the higher plant Sorghum bicolor (non-target organisms). The analysis of the commercial pediculicide showed a good agreement between the contents determined for the two stereoisomers and those labelled in the commercial samples. Different toxic responses and biodegradation profiles were found for each stereoisomer in ecotoxicity assays. The mixture of S/R stereoisomers of bioallethrin resulted more toxic than S-bioallethrin for green algae, with EC50 values of 1.10±0.06 for the mixture and of 1.73±0.05mg/L for the pure S-biallethrin (esbiol). Germination of plants seeds was also affected.

Topics: Allethrins; beta-Cyclodextrins; Chemistry Techniques, Analytical; Chlorophyta; Chromatography, Micellar Electrokinetic Capillary; Deoxycholic Acid; Germination; Insecticides; Limit of Detection; Sorghum; Stereoisomerism

The present work deals with the preparation of innovative cotton textiles which act against blood sucking insects such as mosquitoes. Thus experiments were designed to incorporation of efficient insecticide (Permethrin, bioallethrin) in the macro-molecular structure of modified cotton fabrics. Chemical modification of cotton was realized by grafting with glycidyl methacrylate alone or in combination with β-cyclodextrin by irradiation using fasting electron beam. Retreatment of the so obtained modified cotton was also made to increase the amount of CDs, and in turn, their cavities within the molecular structure of the modified cottons. Finished fabrics were though evaluated using chemical analysis; physical testing, bioassay tests and IR as well as SEM. Results obtained conclude that the amount of insecticide in the finished fabrics increases by increasing of the fixed amount of cyclodextrins which incorporate through their cavities the insecticide. The bioassay test shows that finished cotton fabrics display fast acting against mosquitoes.

Topics: Aedes; Allethrins; Animals; beta-Cyclodextrins; Electrons; Epoxy Compounds; Gossypium; Insecticides; Methacrylates; Permethrin; Polymerization; Radiation, Ionizing; Textiles