betadex and arginyl-glycyl-aspartic-acid

Human mesenchymal stem cells (hMSCs) hold great potential for regenerative medicine. Efficient induction of hMSC differentiation and better understanding of hMSCs behaviors in vitro and in vivo are essential to the clinical translation of stem cell therapy. Here a quantum dots (QDs)-based multifunctional nanoparticle (RGD-β-CD-QDs) is developed for effective enhancing differentiation and long-term tracking of hMSCs in vitro and in vivo. The RGD-β-CD-QDs are modified with β-cyclodextrin (β-CD) and Cys-Lys-Lys-Arg-Gly-Asp (CKKRGD) peptide on the surface. The β-CD can harbor hydrophobic osteogenic small molecule dexamethasone (Dex) and the RGD peptide not only facilitates the complexation of siRNA and delivers siRNA into hMSCs but also leads to cellular uptake of nanoparticles by RGD receptor. Co-delivery of Dex and siRNA by RGD-β-CD-QDs nanocarrier significantly expedites and enhances the osteogenesis differentiation of hMSCs in vitro and in vivo by combined effect of small molecule and RNAi. Furthermore, the RGD-β-CD-QDs can be labeled with hMSCs for a long-term tracking (3 weeks) in vivo to observe the behaviors of implanted hMSCs in animal level. These findings demonstrate that the RGD-β-CD-QDs nanocarrier provides a powerful tool to simultaneously enhance differentiation and long-term tracking of hMSCs in vitro and in vivo for regenerative medicine.

Topics: beta-Cyclodextrins; Cell Differentiation; Humans; Mesenchymal Stem Cells; Oligopeptides; Osteogenesis; Quantum Dots

A quartz crystal microbalance technique with dissipation monitoring and a complementary optical microscopy technique were used for monitoring the capture and release of specific cells on a surface displaying a bifunctional molecular device, composed of a molecular scaffold endowed with the cell recognition property of an RGD ligand and a β-CD/Fc redox-switchable system.

Topics: beta-Cyclodextrins; Cell Separation; Ferrous Compounds; HEK293 Cells; Humans; Integrin alphaVbeta3; Metallocenes; Microscopy; Oligopeptides; Oxidation-Reduction; Peptides, Cyclic; Quartz Crystal Microbalance Techniques; Surface Properties

A novel two-photon absorption (TPA) nanomicelle through the "host-guest" chemistry has been developed and successfully applied in tumor tissue imaging in this work.

Topics: beta-Cyclodextrins; Fluorescent Dyes; HeLa Cells; Humans; MCF-7 Cells; Micelles; Microscopy, Confocal; Nanostructures; Oligopeptides; Photons

A new "plug and play" polymeric template with the driving force of host-guest interaction between β-CD and naphthalene-modified functional groups was designed and studied. Multiple functional groups can be loaded into the template directly and conveniently. Importantly, the "plug and play" effect of the polymeric template is reversible and the functional groups could be removed from the polymeric template conveniently by adding AD-HCl. The studies on the cell viability and phagocytosis proved that the loading and unloading process of this template could be realized in vitro.

Topics: Animals; beta-Cyclodextrins; Camptothecin; Cell Death; Chlorocebus aethiops; COS Cells; Ethylenediamines; HeLa Cells; Humans; Magnetic Resonance Spectroscopy; Microscopy, Confocal; Naphthaleneacetic Acids; Oligopeptides; Peptides; Polymers; Spectrometry, Fluorescence

In this paper, the alpha-beta cyclodextrin dimer is designed via "click" chemistry to connect the hydrophilic and hydrophobic segments to form self-assembled noncovalently connected micelles (NCCMs) through host-guest interactions. A peptide containing the Arg-Gly-Asp (RGD) sequence was introduced to NCCMs as a target ligand to improve the cell uptake efficacy, while PEGylated technology was employed via benzoic-imine bonds to protect the ligands in normal tissues and body fluid. In addition, two fluorescent dyes were conjugated to different segments to track the formation of the micelles as well as the assemblies. It was found that the targeting property of NCCMs was switched off before reaching the tumor sites and switched on after removing the poly(ethylene glycol) (PEG) segment in the tumor sites, which was called "tumor-triggered targeting". With deshielding of the PEG segment, the drugs loaded in NCCMs could be released rapidly due to the thermoinduced phase transition. The new concept of "tumor-triggered targeting" proposed here has great potential for cancer treatment.

Topics: alpha-Cyclodextrins; beta-Cyclodextrins; Cell Survival; Dimerization; Doxorubicin; Drug Carriers; Fluorescein-5-isothiocyanate; Fluorescent Dyes; HeLa Cells; Humans; Hydrophobic and Hydrophilic Interactions; Micelles; Nanoshells; Neoplasms; Oligopeptides; Polyethylene Glycols; Rhodamines