betadex and 3-phenyllactic-acid

Various factors affecting the chiral resolution of 3-phenyllactic acid by capillary zone electrophoresis such as the type of cyclodextrin, concentration of the chiral selector, buffer pH, voltage, and temperature were studied and optimized. Using 30 mmol/L hydroxypropyl-beta-cyclodextrin as the chiral selector, and 0.1 mol/L phosphate solution (pH 5.5) as the running buffer, the baseline separation of 3-phenyllactic acid enantiomers was achieved with a resolution factor of 1.51. The separation voltage was 26 kV and the temperature was 25 degrees C.

Topics: 2-Hydroxypropyl-beta-cyclodextrin; beta-Cyclodextrins; Buffers; Electrophoresis, Capillary; Hydrogen-Ion Concentration; Lactates; Stereoisomerism; Temperature

Uncharged beta-cyclodextrin polymer was used as chiral selector for the enantiomeric separation of some alpha-hydroxy acids by capillary electrophoresis. Complexation and enantiomeric resolution of mandelic acid, m-hydroxy and p-hydroxymandelic acid, 3,4-dihydroxymandelic acid, as well as 2- and 3-phenyllactic acid were studied, changing the concentration of the beta-cyclodextrin polymer added to the background electrolyte at different pH in the range of 4.5-7. Furthermore, the effects of the concentration of the background electrolyte, column temperature, and applied voltage on chiral resolution were also examined. The best enantiomeric separations were obtained using a background electrolyte at pH 6 containing 100 mg/mL of beta-cyclodextrin polymer.

Topics: beta-Cyclodextrins; Cyclodextrins; Electrophoresis, Capillary; Hydrogen-Ion Concentration; Hydroxy Acids; Lactates; Mandelic Acids; Polymers; Stereoisomerism