beta-carotene and retinol-phosphate

Topics: Absorption; Animals; beta Carotene; Biological Transport; Carotenoids; Cell Differentiation; Cell Membrane; Cell Nucleus; Chemical Phenomena; Chemistry; Diterpenes; Homeostasis; Humans; Intracellular Membranes; Polyisoprenyl Phosphate Monosaccharides; Terminology as Topic; Tretinoin; Vitamin A

Adult Brugia pahangi took up and incorporated beta-carotene and free retinol in vitro. The uptake of retinol was 50 times greater than that of beta-carotene under similar incubation conditions. beta-Carotene was almost entirely metabolized, primarily to retinol. The metabolism of retinol by B. pahangi in vitro was less extensive, with a variety of retinoids tentatively identified, including retinyl phosphate (Ret-P), retinyl phosphate mannose (Ret-P-Man) and anhydroretinol as minor metabolites. B. pahangi microsomes were also shown to biosynthesize Ret-P-Man from exogenous Ret-P and GDP-mannose, but not from endogenous lipid acceptors alone. In this circumstance an unidentified lipid appeared to be mannosylated by B. pahangi. The rate of mannose transfer to exogenous Ret-P by B. pahangi microsomes was 150 pmol X min -1. (mg of protein) -1. Ret-P-Man synthetase activity from both B. pahangi and rat liver microsomes had an absolute requirement for bovine serum albumin and MnCl2, and occurred in the absence of detergent. The results suggest a biochemical role for vitamin A in B. pahangi, possibly in filarial glycoprotein synthesis.

Topics: Animals; beta Carotene; Brugia; Carotenoids; Chromatography, Thin Layer; Diterpenes; Filarioidea; Guanosine Diphosphate Mannose; Male; Microsomes; Microsomes, Liver; Polyisoprenyl Phosphate Monosaccharides; Polyisoprenyl Phosphate Sugars; Rats; Rats, Inbred Strains; Vitamin A