benzyloxycarbonylvalyl-alanyl-aspartyl-fluoromethyl-ketone and 10-hydroxycamptothecin

benzyloxycarbonylvalyl-alanyl-aspartyl-fluoromethyl-ketone has been researched along with 10-hydroxycamptothecin* in 1 studies

Other Studies

1 other study(ies) available for benzyloxycarbonylvalyl-alanyl-aspartyl-fluoromethyl-ketone and 10-hydroxycamptothecin

ArticleYear
Induction and mechanism of apoptosis by hydroxycamptothecin in human Tenon's capsule fibroblasts.
    Investigative ophthalmology & visual science, 2012, Jul-24, Volume: 53, Issue:8

    We investigated apoptosis induced by hydroxycamptothecin (HCPT) in human Tenon's capsule using fibroblasts cultured from human Tenon's capsule (HTFs), and the mechanism of induction.. HTFs were treated with 0-4 mg/L HCPT for 24 hours. Cell proliferation was measured by 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT) assay, and apoptotic cells were analyzed by Hoechst 33258 stain. The mRNA and protein levels of caspase-3, -8, and -9 were detected by RT-PCR and Western blotting.. By MTT assay, HCPT induced apoptosis in HTFs in a concentration- and time-dependent manner. Hoechst 33258 staining and transmission electron microscopy showed typical apoptotic morphology, such as condensed chromatin, irregular nuclei, and apoptotic body formation. The mRNA and protein levels of caspase-3 and caspase-9 were upregulated, while caspase-8 was unchanged. Z-VAD-FMK, a caspase inhibitor, inhibited the apoptosis of fibroblasts induced by HCPT. The expression levels of caspase-3 and caspase-9 were down-regulated after Z-VAD-FMK treatment.. Caspase-3 and caspase-9 are important elements in regulating HCPT-induced apoptosis in HTFs.

    Topics: Amino Acid Chloromethyl Ketones; Antineoplastic Agents, Phytogenic; Apoptosis; Blotting, Western; Camptothecin; Caspase 3; Caspase 8; Caspase 9; Cysteine Proteinase Inhibitors; Fibroblasts; Humans; Microscopy, Electron, Transmission; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger; Tenon Capsule

2012
chemdatabank.com