benzofurans and gastrodin

To study the constituents absorbed into blood following the intragstric administration of Dachuanxiong Fang active parts(DCXF) to the experimental migrainous model rats induced by nitroglycerin.. The UPLC-MS was used as the analytic method. The DCXF, Chuanxiong active parts, Tianma active parts, gastrodin, ferulic acid and senkyunolide I were used as the control samples. The information on the total ion chromatogram, mass chromatogram and the mass spectrogram were synthetically analyzed to confirm the constituents absorbed into blood.. Ten of the DCXF constituents were detected in the rats plasma post the intragastric administration of DCXF, in whitch four including gastrodin came from Tianma active parts and six including ferulic acid and senkyunolide I from Chuanxiong active parts.. The findings abtained from the study can provide the useful information for the determination of bioactive substances of the DCXF.

Topics: Animals; Benzofurans; Benzyl Alcohols; Chromatography, High Pressure Liquid; Coumaric Acids; Drugs, Chinese Herbal; Glucosides; Male; Mass Spectrometry; Rats; Rats, Sprague-Dawley