benzofurans and gamma-sitosterol

A new monoterpene (

Topics: A549 Cells; Benzofurans; Cell Cycle Proteins; Drug Screening Assays, Antitumor; HeLa Cells; Humans; Magnetic Resonance Spectroscopy; Molecular Structure; Plant Roots; Saxifragaceae; Sitosterols; Spectrometry, Mass, Electrospray Ionization; Stigmasterol; Transcription Factors; Triterpenes

The underground parts of Aquilegia fragrans are traditionally used for the treatment of wounds and various inflammatory diseases like bovine mastitis. However, there are no reports on the phytochemical characterization and antibacterial studies of A. fragrans.. To isolate compounds from the methanol extract of the underground parts of A. fragrans and determine their antibacterial activity against the pathogens of bovine mastitis. The study was undertaken in order to scientifically validate the traditional use of A. fragrans.. Five compounds were isolated from the methanol extract of the underground parts of A. fragrans using silica gel column chromatography. Structural elucidation of the isolated compounds was done using spectral data analysis and comparison with literature. High performance liquid chromatography (HPLC) was used for the qualitative and quantitative determination of isolated compounds in the crude methanol extract. The methanol extract and isolated compounds were evaluated for antibacterial activities against mastitis pathogens using broth micro-dilution technique.. The five isolated compounds were identified as (1) 2, 4-dihydroxyphenylacetic acid methyl ester (2) β-sitosterol (3) Aquilegiolide (4) Glochidionolactone-A and (5) Magnoflorine. A quick and sensitive HPLC method was developed for the first time for qualitative and quantitative determination of four isolated marker compounds from A. fragrans. The crude methanol extract and compound 5 exhibited weak antibacterial activities that varied between the bacterial species (MIC=500-3000 µg/ml).. The above results show that the crude methanol extract and isolated compounds from A. fragrans exhibit weak antibacterial activities. Further phytochemical and pharmacological studies are required for proper scientific validation of the folk use of this plant species in the treatment of various inflammatory diseases like bovine mastitis.

Topics: Animals; Anti-Bacterial Agents; Aporphines; Aquilegia; Benzofurans; Cattle; Chromatography, High Pressure Liquid; Female; Mastitis, Bovine; Methanol; Microbial Sensitivity Tests; Plant Extracts; Sitosterols

Four new 2,3-secodammarane triterpenoids, stellatonins A-D (3-6), together with a new 3,4-secodammarane triterpenoid, stellatonin E (7), and the known silvestrol (1), 5‴-episilvestrol (2), and β-sitosterol, were isolated from a methanol extract of the stems of Aglaia stellatopilosa through bioassay-guided fractionation. The structures of the new compounds were elucidated using spectroscopic and chemical methods. The compounds were evaluated for their cytotoxic activity against three human cancer cell lines and for their antimicrobial activity using a microtiter plate assay against a panel of Gram-positive and Gram-negative bacteria and fungi.

Topics: Aglaia; Antineoplastic Agents, Phytogenic; Benzofurans; Dammaranes; Drug Screening Assays, Antitumor; Female; Gram-Negative Bacteria; Gram-Positive Bacteria; HT29 Cells; Humans; Malaysia; Microbial Sensitivity Tests; Molecular Structure; Nuclear Magnetic Resonance, Biomolecular; Plant Stems; Saccharomyces cerevisiae; Sitosterols; Triterpenes

Through bioassay-guided fractionation, the EtOAc extract of a culture broth of the endophytic fungus Phoma species ZJWCF006 in Arisaema erubescens afforded a new α-tetralone derivative, (3S)-3,6,7-trihydroxy-α-tetralone (1), together with cercosporamide (2), β-sitosterol (3), and trichodermin (4). The structures of compounds were established on the basis of spectroscopic analyses. Compounds 1, 2, and 3 were obtained from Phoma species for the first time. Additionally, the compounds were subjected to bioactivity assays, including antimicrobial activity, against four plant pathogenic fungi (Fusarium oxysporium, Rhizoctonia solani, Colletotrichum gloeosporioides, and Magnaporthe oryzae) and two plant pathogenic bacteria (Xanthomonas campestris and Xanthomonas oryzae), as well as in vitro antitumor activities against HT-29, SMMC-772, MCF-7, HL-60, MGC80-3, and P388 cell lines. Compound 1 showed growth inhibition against F. oxysporium and R. solani with EC₅₀ values of 413.22 and 48.5 μg/mL, respectively. Additionally, compound 1 showed no cytotoxicity, whereas compound 2 exhibited cytotoxic activity against the six tumor cell lines tested, with IC₅₀ values of 9.3 ± 2.8, 27.87 ± 1.78, 48.79 ± 2.56, 37.57 ± 1.65, 27.83 ± 0.48, and 30.37 ± 0.28 μM, respectively. We conclude that endophytic Phoma are promising sources of natural bioactive and novel metabolites.

Topics: Anti-Bacterial Agents; Antifungal Agents; Antineoplastic Agents; Arisaema; Ascomycota; Benzofurans; Cell Line, Tumor; Culture Media, Conditioned; Endophytes; Fungi; HL-60 Cells; HT29 Cells; Humans; Medicine, Chinese Traditional; Plant Diseases; Sitosterols; Species Specificity; Tetralones; Trichodermin; Xanthomonas

To study the chemical constituents from Hedysarum multijugum.. The compounds were separated by chromatography methods, their structures were identified by spectral analysis.. Six compounds were isolated and identified as beta-sitosterol (1), 7-hydroxy-4'-methoxy isoflavone (2), betulic acid (3), 1,7-dihydroxy-3, 9-dimethoxy pterocarpene (4), 5, 7-dihydroxy-8-C-prenyl-4'-methoxy isoflavone (5) and 5, 7-dihydroxy-4'-methoxy isoflavone (6).. Compound 4 is a new compound and the others were obtained from the plant for the first time.

Topics: Benzofurans; Benzopyrans; Betulinic Acid; Fabaceae; Molecular Structure; Pentacyclic Triterpenes; Plants, Medicinal; Sitosterols; Triterpenes