benzofurans and formic-acid

benzofurans has been researched along with formic-acid* in 2 studies

Other Studies

2 other study(ies) available for benzofurans and formic-acid

ArticleYear
Simultaneous determination of trifolirhizin, (-)-maackiain, (-)-sophoranone, and 2-(2,4-dihydroxyphenyl)-5,6-methylenedioxybenzofuran from Sophora tonkinensis in rat plasma by liquid chromatography with tandem mass spectrometry and its application to a ph
    Journal of separation science, 2014, Volume: 37, Issue:22

    A new liquid chromatography with tandem mass spectrometry method was developed and validated for the simultaneous determination of trifolirhizin, (-)-maackiain, (-)-sophoranone, and 2-(2,4-dihydroxyphenyl)-5,6-methylenedioxybenzofuran from Sophora tonkinensis in rat plasma using chlorpropamide as an internal standard. Plasma samples (50 μL) were prepared using a simple deproteinization procedure with 150 μL of acetonitrile containing 100 ng/mL of chlorpropamide. Chromatographic separation was carried out on an Acclaim RSLC120 C18 column (2.1 × 100 mm, 2.2 μm) using a gradient elution consisting of 7.5 mM ammonium acetate and acetonitrile containing 0.1% formic acid (0.4 mL/min flow rate, 7.0 min total run time). The detection and quantitation of all analytes were performed in selected reaction monitoring mode under both positive and negative electrospray ionization. This assay was linear over concentration ranges of 50-5000 ng/mL (trifolirhizin), 25-2500 ng/mL ((-)-maackiain), 5-250 ng/mL ((-)-sophoranone), and 1-250 ng/mL 2-(2,4-dihydroxyphenyl)-5,6-methylenedioxybenzofuran) with a lower limit of quantification of 50, 25, 5, and 1 ng/mL for trifolirhizin, (-)-maackiain, (-)-sophoranone, and 2-(2,4-dihydroxyphenyl)-5,6-methylenedioxybenzofuran, respectively. All the validation data, including the specificity, precision, accuracy, recovery, and stability conformed to the acceptance requirements. The results indicated that the developed method is sufficiently reliable for the pharmacokinetic study of the analytes following oral administration of Sophora tonkinensis extract in rats.

    Topics: Acetates; Acetonitriles; Animals; Benzodioxoles; Benzofurans; Blood Chemical Analysis; Calibration; Chromatography, Liquid; Flavonoids; Formates; Glucosides; Heterocyclic Compounds, 4 or More Rings; Limit of Detection; Linear Models; Male; Plant Extracts; Pterocarpans; Quality Control; Rats; Rats, Sprague-Dawley; Reproducibility of Results; Sophora; Tandem Mass Spectrometry

2014
Simultaneous determination of lithospermic acid B and its three metabolites by liquid chromatography/tandem mass spectrometry.
    Journal of pharmaceutical and biomedical analysis, 2007, Apr-11, Volume: 43, Issue:5

    A rapid and sensitive method based on liquid chromatography/tandem mass spectrometry (LC/MS/MS) was developed for the simultaneous determination of lithospermic acid B and its three main O-methylated metabolites in rat serum with silibinin as the internal standard. The calibration curves for LSB, and the three metabolites were linear over the ranges of 16-4096, and 8-2048 ng/ml, respectively, with coefficients of correlation >0.998. For LSB, the intra-assay coefficient of variance (CV) was less than 9.3% and the inter-assay CV was less than 8.9%. The inter-assay mean accuracy was between 92.8% and 104.7%. For the three metabolites, the intra-assay CV was less than 8.7% and the inter-assay CV was less than 9.9%. The inter-assay mean accuracy was between 92.5% and 107.9%. This quantitation method was successfully applied to a pharmacokinetic study of LSB in rats. Also, a total recovery of 5.2% was found in bile after oral administration.

    Topics: Absorption; Administration, Oral; Animals; Autoanalysis; Benzofurans; Bile Acids and Salts; Biological Availability; Chromatography, Liquid; Depsides; Drug Stability; Formates; Injections, Intravenous; Methylation; Molecular Structure; Molecular Weight; Rats; Reference Standards; Reproducibility of Results; Sensitivity and Specificity; Silybin; Silymarin; Tandem Mass Spectrometry; Temperature; Time Factors; Water

2007