benzofurans and drimane

Topics: Animals; Anti-Infective Agents; Antineoplastic Agents; Bacillus cereus; Benzofurans; Cell Line, Tumor; Chlorocebus aethiops; Humans; Molecular Structure; Plasmodium falciparum; Polycyclic Sesquiterpenes; Sesquiterpenes; Thailand; Vero Cells; Xylariales

Eight drimane sesquiterpenes (1-8), six isochromane derivatives (9-14), and three known compounds, daldinin B (15), 9alpha-hydroxy-6beta-[(2E,4E,6E)-octa-2,4,6-trienoyloxy]-5alpha-drim-7-en-11,12-olide (16), and pergillin (17), were isolated from the EtOAc extract of the marine-derived fungus Aspergillus ustus 094102. The structures of the new compounds were elucidated on the basis of spectroscopic analysis. The cytotoxic effects on A549 and HL-60 cell lines were evaluated by SRB and MTT methods. Ustusorane E (13) showed significant cytotoxicity against HL-60 cells with an IC50 value of 0.13 microM. Ustusolates C (6) and E (8) exhibited moderate cytotoxicity against A549 and HL-60 cells with IC50 values of 10.5 and 9.0 microM, respectively, and ustusolate A (4) showed weak cytotoxicity against HL-60 and A549 cells with IC50 values of 20.6 and 30.0 microM, respectively.

Topics: Antineoplastic Agents, Phytogenic; Aspergillus; Benzofurans; Chromans; Drug Screening Assays, Antitumor; HL-60 Cells; Humans; Inhibitory Concentration 50; Marine Biology; Molecular Structure; Nuclear Magnetic Resonance, Biomolecular; Polycyclic Sesquiterpenes; Sesquiterpenes; Stereoisomerism