benzofurans and 4-hydroxyphenylpyruvic-acid

Salvia miltiorrhiza is a medicinal plant widely used in the treatment of cardiovascular and cerebrovascular diseases. Hydrophilic phenolic acids, including rosmarinic acid (RA) and lithospermic acid B (LAB), are its primary medicinal ingredients. However, the biosynthetic pathway of RA and LAB in S. miltiorrhiza is still poorly understood. In the present study, we accomplished the isolation and characterization of a novel S. miltiorrhiza Hydroxyphenylpyruvate reductase (HPPR) gene, SmHPPR, which plays an important role in the biosynthesis of RA. SmHPPR contained a putative catalytic domain and a NAD(P)H-binding motif. The recombinant SmHPPR enzyme exhibited high HPPR activity, converting 4-hydroxyphenylpyruvic acid (pHPP) to 4-hydroxyphenyllactic acid (pHPL), and exhibited the highest affinity for substrate 4-hydroxyphenylpyruvate. SmHPPR expression could be induced by various treatments, including SA, GA

Topics: Amino Acid Sequence; Benzofurans; Biosynthetic Pathways; Cinnamates; Depsides; Gene Expression Regulation, Plant; Oxidoreductases; Phenylpropionates; Phenylpyruvic Acids; Phylogeny; Plant Proteins; Plant Roots; Plants, Genetically Modified; Recombinant Proteins; Rosmarinic Acid; Salvia miltiorrhiza; Sequence Alignment