benzofurans and 4-hydroxybenzaldehyde

Studies of the EtOAc extract of the culture broth and methanol extract of the mycelium of Stereum insigne CGMCC5.57 led to the isolation of one new dihydrobenzofuran (1) and six known compounds (2-7). The structures of compounds were elucidated mainly by HRESIMS experiments, and 1D, 2D NMR spectroscopy analysis. This is the first report about the chemical constitutes of the fungus S. insigne.

Topics: Anti-Bacterial Agents; Antinematodal Agents; Bacillus subtilis; Basidiomycota; Benzaldehydes; Benzofurans; Drug Evaluation, Preclinical; Ergosterol; Escherichia coli; Magnetic Resonance Spectroscopy; Molecular Structure; Mycelium; Spectrometry, Mass, Electrospray Ionization

To investigate the chemical constituents of red alga Corallina pilulifera.. Compounds were isolated by normal phase silica gel and Sephadex LH - 20 gel column chromatography, reverse phase HPLC and recrystallization. Their structures were elucidated by spectroscopic methods including MS, 1H-NMR, 13C-NMR, HSQC, HMBC. Cytotoxicity of the compounds was screened by using standard MTT method.. Seven compounds were isolated from red alga C. pilulifera, their structures were identified as (E) -phytol epoxide (1), phytenal (2), phytol (3), dehydrovomifoliol (4), loliolide (5), 3beta-hydroxy-5alpha, 6alpha-epoxy-7-megastigmene-9-one (6), 4-hydroxybenzaldehyde (7).. All of the compounds were obtained from this species for the first time. These compounds were inactive (IC50 > 10 microg x mL(-1)) in the MTT assay.

Topics: Benzaldehydes; Benzofurans; Cell Line, Tumor; Humans; Phytol; Rhodophyta