bay-41-8543 and 3-(5--hydroxymethyl-2--furyl)-1-benzylindazole

bay-41-8543 has been researched along with 3-(5--hydroxymethyl-2--furyl)-1-benzylindazole* in 1 studies

Other Studies

1 other study(ies) available for bay-41-8543 and 3-(5--hydroxymethyl-2--furyl)-1-benzylindazole

Article	Year
The soluble guanylyl cyclase activator YC-1 increases intracellular cGMP and cAMP via independent mechanisms in INS-1E cells. The Journal of pharmacology and experimental therapeutics, 2011, Volume: 338, Issue:3 In addition to increasing cGMP, the soluble guanylyl cyclase (sGC) activator 3-(5'-hydroxymethyl-2'-furyl)-1-benzylindazole (YC-1) can elevate intracellular cAMP levels. This response was assumed to be as a result of cGMP-dependent inhibition of cAMP phosphodiesterases; however, in this study, we show that YC-1-induced cAMP production in the rat pancreatic beta cell line INS-1E occurs independent of its function as a sGC activator and independent of its ability to inhibit phosphodiesterases. This YC-1-induced cAMP increase is dependent upon soluble adenylyl cyclase and not on transmembrane adenylyl cyclase activity. We previously showed that soluble adenylyl cyclase-generated cAMP can lead to extracellular signal-regulated kinase activation and that YC-1-stimulated cAMP production also stimulates extracellular signal-regulated kinase. Although YC-1 has been used as a tool for investigating sGC and cGMP-mediated pathways, this study reveals cGMP-independent pharmacological actions of this compound. Topics: Animals; Blotting, Western; Cell Line; Cyclic AMP; Cyclic GMP; Enzyme Activators; Enzyme Inhibitors; Glucose; Guanylate Cyclase; Indazoles; Insulin-Secreting Cells; Mitogen-Activated Protein Kinases; Morpholines; Oxadiazoles; Oxazines; Phosphoric Diester Hydrolases; Pyrimidines; Quinoxalines; Rats; Transcription, Genetic	2011

Article

Year

The soluble guanylyl cyclase activator YC-1 increases intracellular cGMP and cAMP via independent mechanisms in INS-1E cells.

The Journal of pharmacology and experimental therapeutics, 2011, Volume: 338, Issue:3

In addition to increasing cGMP, the soluble guanylyl cyclase (sGC) activator 3-(5'-hydroxymethyl-2'-furyl)-1-benzylindazole (YC-1) can elevate intracellular cAMP levels. This response was assumed to be as a result of cGMP-dependent inhibition of cAMP phosphodiesterases; however, in this study, we show that YC-1-induced cAMP production in the rat pancreatic beta cell line INS-1E occurs independent of its function as a sGC activator and independent of its ability to inhibit phosphodiesterases. This YC-1-induced cAMP increase is dependent upon soluble adenylyl cyclase and not on transmembrane adenylyl cyclase activity. We previously showed that soluble adenylyl cyclase-generated cAMP can lead to extracellular signal-regulated kinase activation and that YC-1-stimulated cAMP production also stimulates extracellular signal-regulated kinase. Although YC-1 has been used as a tool for investigating sGC and cGMP-mediated pathways, this study reveals cGMP-independent pharmacological actions of this compound.

Topics: Animals; Blotting, Western; Cell Line; Cyclic AMP; Cyclic GMP; Enzyme Activators; Enzyme Inhibitors; Glucose; Guanylate Cyclase; Indazoles; Insulin-Secreting Cells; Mitogen-Activated Protein Kinases; Morpholines; Oxadiazoles; Oxazines; Phosphoric Diester Hydrolases; Pyrimidines; Quinoxalines; Rats; Transcription, Genetic

2011