bardoxolone-methyl and corilagin

The Holliday junction (HJ) branch migrator RuvAB complex plays a fundamental role during homologous recombination and DNA damage repair, and therefore, is an attractive target for the treatment of bacterial pathogens. Pseudomonas aeruginosa (P. aeruginosa, Pa) is one of the most common clinical opportunistic bacterial pathogens, which can cause a series of life-threatening acute or chronic infections. Here, we performed a high throughput small-molecule screening targeting PaRuvAB using the FRET-based HJ branch migration assay. We identified that corilagin, bardoxolone methyl (BM) and 10-(6'-plastoquinonyl) decyltriphenylphosphonium (SKQ1) could efficiently inhibit the branch migration activity of PaRuvAB, with IC

Topics: Adenosine Triphosphate; Bacterial Proteins; DNA Helicases; DNA Repair; DNA-Binding Proteins; DNA, Bacterial; DNA, Cruciform; Escherichia coli; Escherichia coli Proteins; Glucosides; Hydrolyzable Tannins; Molecular Docking Simulation; Oleanolic Acid; Pseudomonas aeruginosa; Recombination, Genetic