bambermycins and tetramethylrhodamine

bambermycins has been researched along with tetramethylrhodamine* in 1 studies

Other Studies

1 other study(ies) available for bambermycins and tetramethylrhodamine

Article	Year
Fluorescence correlation spectroscopy (FCS)-based characterisation of aptamer ligand interaction. Methods in molecular biology (Clifton, N.J.), 2009, Volume: 535 Fluorescence correlation spectroscopy (Bacia and Schwille (2007) Nat. Protoc. 2, 2842-2856) reveals molecular mobilities, enabling to identify molecular interactions based on a change of diffusion times (Rigler and Elson, (2001) Fluorescence Correlation Spectroscopy: Theory and Applications. Springer, Berlin; Haustein, and Schwille, (2004) Curr. Opin. Struct. Biol. 14, 531-540). This technique can be applied to determine the dissociation constant of a complex formed by a fluorescence-labelled target and its corresponding RNA aptamer selected via systematic evolution of ligands by exponential enrichment (SELEX) (Schürer, et al. (2001) Biol. Chem. 382, 47948). Here, an FCS titration experiment is described in detail, where the binding properties of tetramethylrhodamine (TMR) labelled Moenomycin A to its corresponding RNA aptamer were determined (Schürer, et al. (2001) Biol. Chem. 382, 47948). Topics: Aptamers, Nucleotide; Bambermycins; Binding Sites; Fluorescent Dyes; Kinetics; Ligands; Rhodamines; SELEX Aptamer Technique; Spectrometry, Fluorescence; Titrimetry	2009

Article

Year

Fluorescence correlation spectroscopy (FCS)-based characterisation of aptamer ligand interaction.

Methods in molecular biology (Clifton, N.J.), 2009, Volume: 535

Fluorescence correlation spectroscopy (Bacia and Schwille (2007) Nat. Protoc. 2, 2842-2856) reveals molecular mobilities, enabling to identify molecular interactions based on a change of diffusion times (Rigler and Elson, (2001) Fluorescence Correlation Spectroscopy: Theory and Applications. Springer, Berlin; Haustein, and Schwille, (2004) Curr. Opin. Struct. Biol. 14, 531-540). This technique can be applied to determine the dissociation constant of a complex formed by a fluorescence-labelled target and its corresponding RNA aptamer selected via systematic evolution of ligands by exponential enrichment (SELEX) (Schürer, et al. (2001) Biol. Chem. 382, 47948). Here, an FCS titration experiment is described in detail, where the binding properties of tetramethylrhodamine (TMR) labelled Moenomycin A to its corresponding RNA aptamer were determined (Schürer, et al. (2001) Biol. Chem. 382, 47948).

Topics: Aptamers, Nucleotide; Bambermycins; Binding Sites; Fluorescent Dyes; Kinetics; Ligands; Rhodamines; SELEX Aptamer Technique; Spectrometry, Fluorescence; Titrimetry

2009