bacillithiol and malic-acid

The first step during bacillithiol (BSH) biosynthesis involves the formation of N-acetylglucosaminylmalate from UDP-N-acetylglucosamine and l-malate and is catalyzed by a GT4 class glycosyltransferase enzyme (BshA). Recombinant Staphylococcus aureus and Bacillus subtilis BshA were highly specific and active with l-malate but the former showed low activity with d-glyceric acid and the latter with d-malate. We show that BshA is inhibited by BSH and similarly that MshA (first enzyme of mycothiol biosynthesis) is inhibited by the final product MSH.

Topics: Antioxidants; Bacillaceae Infections; Bacillus subtilis; Bacterial Proteins; Cysteine; Enzyme Inhibitors; Glucosamine; Glycopeptides; Inositol; Kinetics; Malates; Models, Molecular; Molecular Targeted Therapy; Molecular Weight; N-Acetylglucosaminyltransferases; Protein Conformation; Recombinant Proteins; Staphylococcal Infections; Staphylococcus aureus; Substrate Specificity; Uridine Diphosphate N-Acetylglucosamine