azoxystrobin and sulfoxaflor

Pesticides pose a potential threat to bee health, especially in combination with other stressors, such as parasites. However, pesticide risk assessment tests pesticides in isolation from other stresses, i.e., on otherwise healthy bees. Through molecular analysis, the specific impacts of a pesticide or its interaction with another stressor can be elucidated. Molecular mass profiling by MALDI BeeTyping

Topics: Animals; Bees; Host-Parasite Interactions; Parasites; Pesticides; Proteome

The gut microbiome plays an important role in bee health and disease. But it can be disrupted by pesticides and in-hive chemicals, putting honey bee health in danger. We used a controlled and fully crossed laboratory experimental design to test the effects of a 10-day period of chronic exposure to field-realistic sublethal concentrations of two nicotinic acetylcholine receptor agonist insecticides (nACHRs), namely flupyradifurone (FPF) and sulfoxaflor (Sulf), and a fungicide, azoxystrobin (Azoxy), individually and in combination, on the survival of individual honey bee workers and the composition of their gut microbiota (fungal and bacterial diversity). Metabarcoding was used to examine the gut microbiota on days 0, 5, and 10 of pesticide exposure to determine how the microbial response varies over time; to do so, the fungal ITS2 fragment and the V4 region of the bacterial 16S rRNA were targeted. We found that FPF has a negative impact on honey bee survival, but interactive (additive or synergistic) effects between either insecticide and the fungicide on honey bee survival were not statistically significant. Pesticide treatments significantly impacted the microbial community composition. The fungicide Azoxy substantially reduced the Shannon diversity of fungi after chronic exposure for 10 days. The relative abundance of the top 10 genera of the bee gut microbiota was also differentially affected by the fungicide, insecticides, and fungicide-insecticide combinations. Gut microbiota dysbiosis was associated with an increase in the relative abundance of opportunistic pathogens such as Serratia spp. (e.g. S. marcescens), which can have devastating consequences for host health such as increased susceptibility to infection and reduced lifespan. Our findings raise concerns about the long-term impact of novel nACHR insecticides, particularly FPF, on pollinator health and recommend a novel methodology for a refined risk assessment that includes the potential effects of agrochemicals on the gut microbiome of bees.

Topics: 4-Butyrolactone; Animals; Bees; Fungicides, Industrial; Gastrointestinal Microbiome; Insecticides; Pesticides; Pyridines; Pyrimidines; Receptors, Nicotinic; RNA, Ribosomal, 16S; Serratia marcescens; Strobilurins; Sulfur Compounds

Exposure to pesticides is considered a major threat to bees and several neonicotinoid insecticides were recently banned in cropland within the European Union in light of evidence of their potential detrimental effects. Nonetheless, bees remain exposed to many pesticides whose effects are poorly understood. Recent evidence suggests that one of the most prominent replacements of the banned neonicotinoids - the insecticide sulfoxaflor - harms bees and that fungicides may have been overlooked as a driver of bee declines. Realistic-exposure studies are, however, lacking. Here, we assess the impact of the insecticide Closer (active ingredient: sulfoxaflor) and the widely used fungicide Amistar (a.i.: azoxystrobin) on honeybees in a semi-field study (10 flight cages containing a honeybee colony, for each of three treatments: Closer, Amistar, control). The products were applied according to label instructions either before (Closer) or during (Amistar) the bloom of purple tansy. We found no significant effects of Closer or Amistar on honeybee colony development or foraging activity. Our study suggests that these pesticides pose no notable risk to honeybees when applied in isolation, following stringent label instructions. The findings on Closer indicate that a safety-period of 5-6 days between application and bloom, which is only prescribed in a few EU member states, may prevent its impacts on honeybees. However, to conclude whether Closer and Amistar can safely be applied, further realistic-exposure studies should examine their effects in combination with other chemical or biological stressors on various pollinator species.

Topics: Animals; Bees; Fungicides, Industrial; Insecticides; Neonicotinoids; Pyridines; Pyrimidines; Strobilurins; Sulfur Compounds