azaserine has been researched along with carbene* in 2 studies
2 other study(ies) available for azaserine and carbene
Article | Year |
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Complete integration of carbene-transfer chemistry into biosynthesis.
Biosynthesis is an environmentally benign and renewable approach that can be used to produce a broad range of natural and, in some cases, new-to-nature products. However, biology lacks many of the reactions that are available to synthetic chemists, resulting in a narrower scope of accessible products when using biosynthesis rather than synthetic chemistry. A prime example of such chemistry is carbene-transfer reactions Topics: Azaserine; Biocatalysis; Biological Products; Coenzymes; Cyclopropanes; Cytochrome P-450 Enzyme System; Multigene Family; Styrene | 2023 |
Discovery of the Azaserine Biosynthetic Pathway Uncovers a Biological Route for α-Diazoester Production.
Azaserine is a bacterial metabolite containing a biologically unusual and synthetically enabling α-diazoester functional group. Herein, we report the discovery of the azaserine (aza) biosynthetic gene cluster from Glycomyces harbinensis. Discovery of related gene clusters reveals previously unappreciated azaserine producers, and heterologous expression of the aza gene cluster confirms its role in azaserine assembly. Notably, this gene cluster encodes homologues of hydrazonoacetic acid (HYAA)-producing enzymes, implicating HYAA in α-diazoester biosynthesis. Isotope feeding and biochemical experiments support this hypothesis. These discoveries indicate that a 2-electron oxidation of a hydrazonoacetyl intermediate is required for α-diazoester formation, constituting a distinct logic for diazo biosynthesis. Uncovering this biological route for α-diazoester synthesis now enables the production of a highly versatile carbene precursor in cells, facilitating approaches for engineering complete carbene-mediated biosynthetic transformations in vivo. Topics: Azaserine; Biosynthetic Pathways; Methane; Multigene Family; Oxidation-Reduction | 2023 |