azadirachtin and tebufenozide

Azadirachtin, as the most promising and effective botanical insecticide, exhibits significant growth inhibition activity against agricultural and forestry pests. However, its biochemical effects at the metabolic level compared with those of other insect growth regulators have not been studied. Therefore, in this study, a GC-MS based untargeted metabolomics approach was applied to compare azadirachtin with pyriproxyfen (a juvenile hormone analog) and tebufenozide (a molting hormone analog) in terms of their metabolic effects on Bactrocera dorsalis larvae. The bioactivity of azadirachtin against B. dorsalis larvae was significantly different than those of pyriproxyfen and tebufenozide. A total of 693 mass features were recognized, and 112 metabolites were identified in this study. The results showed that a total of 16, 13 and 10 differentially regulated metabolites corresponding to 12, 5 and 8 pathways occur in Aza versus CK, Pyr versus CK and Teb versus CK group, respectively. Further analysis showed that 6 differentially regulated metabolites corresponding to 5 key pathways could be the primary differential metabolic response of B. dorsalis larvae to the three insect growth regulators. The pathways were myo-inositol corresponding to ascorbate and aldarate metabolism as the specific response of B. dorsalis larvae to azadirachtin; xylitol, xylulose and 3-aminopropionitrile corresponding to pentose and glucuronate interconversions, and cyanoamino acid metabolism as the common responses to azadirachtin and pyriproxyfen; and 3-hydroxypropionic acid and beta-alanine corresponding to propanoate metabolism and beta-alanine metabolism as the specific responses to tebufenozide. The results showed that the metabolic response of B. dorsalis larvae to azadirachitin is closer to that of pyriproxyfen than tebufenozide. The differentially regulated metabolites and pathways responsible for this difference are discussed.

Topics: Animals; Hydrazines; Insect Hormones; Insecticides; Larva; Limonins; Metabolome; Metabolomics; Pyridines; Tephritidae

The insect development is intricately controlled by morphogenetic hormones, juvenile hormone (JH) and 20-hydroxyecdysone (20E) through the regulation of gene/protein expression. The role of hexamerins in the metamorphosis of insects and reproduction and their control by 20E at the gene level has been widely reported in insects. In the present study we for the first time report the role of ecdysteroids in the regulation of hexamerin synthesis in a lepidopteran insect Corcyra cephalonica. The hormonal studies were carried out using the normal and the thorax-ligated insects with both 20E and its non-steroidal agonist RH-5992. The in vitro as well as in vivo studies showed a stimulatory effect of 20E and its agonist on the hexamerin synthesis including arylphorin (Hex 2), whereas hormone blockade with azadirachtin caused a time dependent reduction in synthesis. The northern analysis using Hex 2b cDNA as probe too confirmed the above result. This was followed by the cloning of the Hex 2b gene. The full length of the genomic clone was found to be 3.5kb long and has four exons interspersed by three introns. The genome walking analysis revealed the presence of a steroid hormone binding sequence "Ecdysone response element" (EcRE) in the 5' untranscribed region (UTR) of the gene. The data presented in this paper clearly suggest that hexamerin synthesis in C. cephalonica is transcriptionally regulated by 20E.

Topics: Animals; Base Sequence; Blotting, Northern; Cloning, Molecular; DNA Primers; Ecdysterone; Electrophoresis, Polyacrylamide Gel; Gene Components; Gene Expression Regulation; Hydrazines; Immunoprecipitation; Insect Proteins; Insecticides; Limonins; Metamorphosis, Biological; Molecular Sequence Data; Moths; Polymerase Chain Reaction; Reproduction; Response Elements; Sequence Analysis, DNA

This study assessed the effects of exposure to IGRs on the long-term development of the honeybee colony, viability of queens and sperm production in drones and integrated the data into a honeybee population model. Colonies treated with diflubenzuron resulted in a short-term reduction in the numbers of adult bees and brood. Colonies treated with fenoxycarb declined during the season earlier and started the season slower. The number of queens that successfully mated and laid eggs was affected in the fenoxycarb treatment group but there were no significant differences in the drone sperm counts between the colonies. An existing honeybee population model was modified to include exposure to IGRs. In the model, fenoxycarb reduced the winter size of the colony, with the greatest effects following a June or an August application. Assuming a 'larvae per nurse bee' ratio of 1.5 for brood rearing capability, the reduction in winter size of a colony following a fenoxycarb application was at its worst about 8%. However, even if only those bees reared within 2 weeks of the IGR being applied are subject to premature ageing, this might significantly reduce the size of over-wintering colonies, and increase the chance of the bee population dwindling and dying in late winter or early spring.

Topics: Animals; Bees; Chitin; Diflubenzuron; Ecdysteroids; Female; Hydrazines; Insecticides; Limonins; Male; Phenylcarbamates; Population Density; Reproduction; Sperm Count