atranol and methyl-orsellinate

To develop a RP-HPLC method for determining the contents of atranol, lecanorin, ethyl orsellinate and methyl orsellinate in Usnea diffracta.. A Kromasil-C18 column (4.6 mm x 250 mm, 5 microm) was used at 25 degrees C with the mobile phases of acetonitrile -1% acetic acid in a gradient manner. The flow rate was set at 1.0 mL x min(-1). The detection wavelength was 280 nm.. The correlation coefficients of atranol, lecanorin, ethyl orsellinate, and methyl orsellinate were higher than 0.999. Recoveries were from 102.9% to 95.30%; with RSD from 2.3% to 1.9%.. The method is quick, simple and repeatable for simultaneous determination of atranol, lecanorin, ethyl orsellinate and methyl orsellinate in U. diffracta.

Topics: Benzaldehydes; Chromatography, High Pressure Liquid; Resorcinols; Usnea