astatine and sodium-perchlorate

The cellular expression of the sodium iodide symporter (NIS) has been shown to confer iodide-concentrating capacity in non-thyroid cell types. We examined the role of NIS in the uptake of the alpha-particle emitting radiohalide [(211)At]astatide in the UVW human glioma cell line transfected to express NIS. [(211)At]Astatide uptake is shown to be NIS-dependent, with characteristics similar to [(131)I]iodide uptake. These studies suggest [(211)At]astatide as a possible alternative radionuclide to [(131)I]iodide for NIS-based endoradiotherapy, and provide a model for the study of [(211)At]astatide behavior at a cellular level.

Topics: Astatine; Dose-Response Relationship, Drug; Glioma; Humans; Iodine Radioisotopes; Methimazole; Perchlorates; Radiopharmaceuticals; Reference Values; Reproducibility of Results; Sensitivity and Specificity; Sodium Compounds; Symporters; Transfection; Tumor Cells, Cultured

The transport and accumulation of free 211At and 125I- were investigated in thyrocytes cultured as monolayers in bicameral chambers under the influence of thyroid-stimulating hormone, stable iodide, ouabain and perchlorate. The results indicate that there are similarities and differences in the transport mechanisms of free 211At and 125I-. These results will be valuable in the development of radiation protection when handling and using 211At-labeled radiopharmaceuticals, and for the potential use of free 211At in radiation therapy of poorly differentiated thyroid cancer.

Topics: Animals; Astatine; Biological Transport; Cell Culture Techniques; Cells, Cultured; Enzyme Inhibitors; Epithelium; Iodine Radioisotopes; Ouabain; Perchlorates; Sodium Compounds; Swine; Thyroid Gland; Thyrotropin