ascorbic-acid and triethyllead

The effect of triethyllead (TriEL) on motile activity, structure of cytoskeleton and chemotaxis of Dictyostelium discoideum amoebae in developing concentration gradients of folic acid (FA) and cAMP has been studied. It was observed that 3 microM TriEL had little or no effect on locomotion and chemotactic response of cells, whereas 5 microM TriEL strongly reduced the motile activity of Dictyostelium discoideum amoebae and inhibited their chemotaxis towards cAMP, but not towards FA. FA was found to restore the motile activity of Dictyostelium discoideum, inhibited by TriEL. A similar effect was observed in the presence of other antioxidants, i.e. ascorbic acid and sodium selenite, suggesting that oxidative stress may be involved in the action of TriEL. Moreover, the treatment of Dictyostelium amoebae with 5 microM TriEL caused disruption of microtubules while 3 microM TriEL had little effect on their structure. FA caused restoration of microtubules only in some cells within 1 h of incubation, i.e. when the directional movement of cells towards this chemoattractant was already observed. However, their organization was significantly different from that observed in the untreated cells, suggesting that microtubule undisturbed organisation may be not necessary for Dictyostelium discoideum amoebae locomotion and chemotaxis

Topics: Actins; Animals; Ascorbic Acid; Cell Movement; Cells, Cultured; Chemotactic Factors; Chemotaxis; Cyclic AMP; Dictyostelium; Fluorescent Antibody Technique; Folic Acid; Microtubules; Organometallic Compounds; Sodium Selenite