ascorbic-acid and tocotrienol--alpha

Antioxidants, including vitamin E, may have a positive effect on human health and prolong storage of food items. Vitamin E content and antioxidant capacity were measured in 25 barley genotypes before and after 4 months storage at 10 °C using high performance liquid chromatography (HPLC) and ability to scavenge DPPH radicals, respectively. As expected, α-tocotrienol (α-T3) and α-tocopherol (α-T) were the predominant tocol isomers. Vitamin E content and antioxidant capacity varied significantly among genotypes. Vitamin E ranged from 8.5 to 31.5 μg/g dry weight (DW) while ascorbic acid equivalent antioxidant capacity (AEAC) varied from 57.2 to 158.1 mg AEAC/100 g fresh weight (FW). Generally, lower vitamin E content or antioxidant capacity was observed in hulless or coloured genotypes. These results suggest that some genotypes are potential candidates for breeding of barley cultivars with high vitamin E content or antioxidant capacity at harvest, even after storage.

Topics: alpha-Tocopherol; Antioxidants; Ascorbic Acid; Food Storage; Genotype; Hordeum; Tocotrienols; Vitamin E

Topics: Animals; Antioxidants; Ascorbic Acid; Electron Spin Resonance Spectroscopy; Erythrocyte Membrane; Free Radical Scavengers; Free Radicals; Hexanes; Humans; Indicators and Reagents; Iron; Kinetics; Lipid Peroxidation; Liposomes; Microsomes; Mitochondria, Heart; Myocardium; Rats; Superoxides; Tocotrienols; Vitamin E