ascorbic-acid and saccharolactone

The role of aromatic hydrocarbon receptor (AhR)-mediated signal transduction pathways was investigated in the regulation of ascorbate synthesis by using Ah-responsive and Ah-unresponsive mouse strains. In vivo 3-methylcholanthrene treatment increased hepatic and plasma ascorbate concentrations only in the Ah-responsive strain. The mRNA level of gulonolactone oxidase and the microsomal ascorbate production from p-nitrophenyl glucuronide, D-glucuronic acid or gulonolactone in the liver of Ah-responsive and Ah-unresponsive mice were compared. In Ah-responsive mice, these parameters were higher originally, and they further increased upon in vivo addition of 3-methylcholanthrene, while in Ah-unresponsive mice the treatment was not effective. These results suggest that the transcription of gulonolactone oxidase gene is regulated by an Ah receptor-dependent signal transduction pathway.

Topics: Animals; Ascorbic Acid; Cytochrome P-450 CYP1A1; Glucaric Acid; Glucuronates; Glucuronic Acid; Glucuronides; Glucuronosyltransferase; L-Gulonolactone Oxidase; Male; Methylcholanthrene; Mice; Mice, Inbred Strains; Microsomes, Liver; Receptors, Aryl Hydrocarbon; RNA, Messenger; Signal Transduction; Sugar Alcohol Dehydrogenases; Time Factors