ascorbic-acid and potassium-bromide

We describe the synthesis, properties, and application of two reversible fluorescent probes, mCy-TemOH and Cy-TemOH, for HOBr sensing and imaging in live cells. The two probes contain a hydroxylamine functional group for the monitoring of HOBr oxidation/ascorbic acid reduction events. Confocal fluorescence microscopy has established the HOBr detection in live-cells.

Topics: Animals; Ascorbic Acid; Bromates; Bromides; Cell Line; Cyclic N-Oxides; Eosinophil Peroxidase; Fluorescent Dyes; Hydrogen Peroxide; Hydroxylamines; Indoles; Macrophages; Mice; Microscopy, Confocal; Molecular Imaging; Oxidation-Reduction; Potassium Compounds; Spectrometry, Fluorescence

Stable Pd nanocubes (PdNC) with the average size ~15 nm were prepared by the controlled reduction of sodium tetrachloropalladate with ascorbic acid in water, in the presence of polyvinylpyrrolidone and potassium bromide. Morphology of the particles was characterized by transmission electron microscopy (TEM) and their stability in the colloidal solution was verified by dynamic light scattering (DLS). It has been demonstrated that the Pd nanocubes can be distinguished from commercial Au nanospheres in a standard TEM microscope by means of automated image analysis. In the next step, the PdNC were successfully conjugated to immunoglobulin proteins and used for the detection of a specific protein (nucleophosmin) on ultrathin sections of HeLa cells. Our experiments showed that PdNC can be used for multiple immunolabeling in combination with commercial Au nanospheres.

Topics: Ascorbic Acid; Bromides; Colloids; HeLa Cells; Humans; Image Processing, Computer-Assisted; Immunoconjugates; Immunoglobulins; Metal Nanoparticles; Microscopy, Electron, Transmission; Microtomy; Nuclear Proteins; Nucleophosmin; Palladium; Particle Size; Potassium Compounds; Povidone; Staining and Labeling; Water

The potassium bromate (PB) is the flour improving additive for bread making, most widely used in Venezuela. This additive has been reported to have hazardous effects. For this reason it has been forbidden in various countries. In order to find a substitute for PB, the feasibility of using ascorbic acid (AA) in bread making flours was considered. Flours with 80, 40 and 20 ppm of AA were tested and contrasted with an experimental and industrial flour with 80 ppm of PB, maximum quantity allowed by the Venezuelan Legislation. The effect of these additives was evaluated on French bread, of high consumption in our country. It was found that the bread prepared using the flour containing 20 ppm of AA did not present significant differences from those made out of the pattern flour, concerning their organoleptic and physico-chemical properties. It was demonstrated that it is technically feasible to replaced 80 ppm of PB for 20 ppm of AA in the flours, without affecting the bread acceptability.

Topics: Animals; Ascorbic Acid; Bread; Bromides; Carcinogens; Feasibility Studies; Flour; Food Additives; Food Preferences; Humans; Legislation, Food; Potassium Compounds; Rats; Venezuela