ascorbic-acid and hydroxide-ion

The cytotoxic metabolites of oxygen [superoxide (O-2), hydrogen peroxide (H2O2), and hydroxyl (OH.)] have been demonstrated to be involved in the peroxidation of membrane lipids consequently altering membrane composition, morphology, and function. Of all the lines of defense adopted by living organisms against toxic oxygen free radicals, vitamin E is most effective in the prevention of membrane damage. Cardiopulmonary bypass (CPB) has been shown to activate complement and cause sequestration of leukocytes which can recruit, adhere, and stimulate release of cytotoxic oxygen radicals. A prospective study of 30 patients evaluated the effects of CPB with and without an exogenous free radical scavenger (Group I, N = 20, control) and (Group II, N = 10, vitamin E) on H2O2 (a marker of oxygen free radicals) malonaldehyde (a marker of lipid peroxidation), transpulmonary leukosequestration, and plasma levels of vitamins E and C. Group I showed a progressive increase in H2O2 during CPB from 65 +/- 6 to 130 +/- 11 micron/ml (P less than 0.0001); plasma vitamin E decreased from 15 +/- 3 to 6 +/- 1 mg/liter (P less than 0.0001) while vitamin C increased from 1.6 +/- .3 to 2.3 +/- .3 mg/dl (P less than 0.0001). Group II showed no significant increase in H2O2 (from 78 +/- 8 to 93 +/- 5 microns/ml) during CPB and a significant reduction in H2O2 levels compared to Group I (P less than 0.001); plasma vitamins E and C did not change significantly in Group II.(ABSTRACT TRUNCATED AT 250 WORDS)

Topics: Ascorbic Acid; Cardiopulmonary Bypass; Clinical Trials as Topic; Female; Free Radicals; Humans; Hydrogen Peroxide; Hydroxides; Leukocytes; Lung; Male; Middle Aged; Oxygen; Random Allocation; Superoxides; Vitamin E

The major obstacle to developing nanozymes which are considered as promising alternatives to natural enzymes is their moderate performance, including poor affinity for substrates, low catalytic activity, and severe pH-dependence. To address these issues, herein, we synthesize ultrathin layered double hydroxide (LDH) nanosheets with a thickness of 1.4 nm and an average lateral size of 23 nm using a fast-precipitation method. Through the rational design of their compositions, it is found that NiMn LDHs exhibit the optimum peroxidase mimicking performance with excellent substrate affinity, high catalytic activity (a limit of detection (LOD) of 0.04 μM H

Topics: Ascorbic Acid; Biological Assay; Biosensing Techniques; Horseradish Peroxidase; Hydrogen Peroxide; Hydroxides; Manganese; Nanoparticles; Nickel; Paper; Particle Size; Surface Properties

A bionanohybrid based on ascorbic acid-intercalated layered double hydroxides (LDHs) was synthesized using a facile and novel mechanochemical grinding technique, and its efficacy as an edible food coating is reported. Ascorbic acid-intercalated Mg-Al-LDHs (AA-LDHs) are synthesized using a green water-assisted grinding approach. The successful synthesis of the mechanochemically ground AA-LDHs was confirmed by the shifts observed in the basal peaks of the LDHs based on a powder X-ray diffraction, changes in the positions of vibrational frequencies of ascorbic acid based on Fourier Transform Infrared Spectroscopy, and significant changes in the intensity and peak positions of the core-shell bands based on X-ray photoelectron spectroscopy. The resulting nanohybrid further demonstrates thermal stability in thermogravimetric and derivative thermogravimetric analysis. Transmission electron microscopy images of the mechanochemically synthesized AA-LDHs reveal a plate-like morphology, which is a characteristic of the hydrotalcite-like structure. In a novel application, an edible coating was prepared by blending the AA-LDHs into a biocompatible alginate matrix, and the coating was developed on freshly plucked strawberries using the dip-coating method. In order to evaluate the efficacy of the coating, the total phenolic content, pH, microbial growth, weight loss, titratable acidity, and ascorbic acid content were monitored in the coated and uncoated fruits for a period of 18 days. The results reveal that the shelf life of strawberries increases from 9 days to 15 days for the nanohybrid coated fruits, suggesting the potential food preservation applications of the nanohybrid.

Topics: Alginates; Ascorbic Acid; Biomechanical Phenomena; Drug Compounding; Food Preservation; Food Preservatives; Food Storage; Fragaria; Fruit; Hydroxides; Nanostructures; Spectroscopy, Fourier Transform Infrared

The attendant side effects associated with some synthetic drugs used in the management of diseases have led to the search for safer alternative therapies that are relatively cheaper with minimal side effects.. The methanol extract of Calliandra portoricensis root bark (CPRB) was orally administered at the doses of 5, 10, 20, and 25 mg/kg body weight for 14 consecutive days of 5 rats in each group. The control rats were given distilled water.. The 95% methanol extract of CPRB significantly (p<0.05) scavenged NO• and OH• radicals compared to vitamin C. The level of lipid peroxidative products (malondialdehyde, MDA) was significantly (p<0.05) attenuated in a dose-dependent manner. Antioxidant enzymes including superoxide dismutase and catalase were significantly (p<0.05) exercabated in both liver and kidney in a dose-dependent manner. Furthermore, serum AST, alanine aminotransaminase and γ-glutamyltransferase (GGT) activity depicted non-significant (p>0.05) increase in the treated animals. The histological examination showed mild vacuolar, portal congestion and cell infiltration by mononuclear of the hepatic tissues.. The study then concluded that a therapeutic dose of the methanol extract of CPRB triggered the antioxidant defence systems in male rats. It is, therefore, recommended that the doses should be carefully and clinically chosen because higher doses may cause some health risks.

Topics: Animals; Antioxidants; Ascorbic Acid; Disease Models, Animal; Dose-Response Relationship, Drug; Fabaceae; Free Radical Scavengers; Hydroxides; Inhibitory Concentration 50; Kidney; Lipid Peroxidation; Liver; Male; Malondialdehyde; Nitric Oxide; Plant Extracts; Plant Roots; Rats; Rats, Wistar

A new drug delivery system for vitamin C (VC), Ca/Al layered double hydroxide (LDH), is demonstrated in this work. VC anions were intercalated successfully in the Ca/Al LDH gallery by a coprecipitation method. The interlayer space of 9.8Å suggests that VC anions are vertical to the LDH layers in the form of interdigitated bilayer. The loading of VC in LDH is 36.4wt.%. The thermal stability of VC is significantly enhanced after intercalation. In vitro VC release results show that the release time of VC in a phosphate buffer at pH7.4 was significantly extended, and the maximal percentage of VC released is 80% of the total. The Avrami-Erofe'ev equation most satisfactorily explains the release kinetics of VC, which is that the release of VC is mainly dominated by the ion-exchange reaction.

Topics: Aluminum; Ascorbic Acid; Calcium; Delayed-Action Preparations; Drug Carriers; Hydrogen-Ion Concentration; Hydroxides; Intercalating Agents; Nanoparticles; Spectroscopy, Fourier Transform Infrared; Surface Properties; X-Ray Diffraction

A new method for calculating theoretical bond dissociation enthalpy (BDE) and bond dissociation free energy (BDFE) of hydroxylic antioxidants is forwarded. BDE and BDFE may be understood as activation energies accompanying the formation of transition states, which may undergo downhill homolytic dissociation. The new method does not involve the complete fission of O-H bonds.. Theoretical gas phase BDE values were calculated with the ab initio unrestricted Hartree-Fock (UHF) method, as changes in enthalpy between ground singlet states (GS) and triplet dissociative states (DS). Similarly, gas phase BDFEs were estimated from the corresponding changes in Gibbs free energy. The results were then compared with reliable experimental reports.. The proposed theoretical approach of BDE and BDFE determination was tested using 10 simple phenols, 5 flavonoids, and l-ascorbic acid derivatives. The agreement between our calculated gas phase results and the adopted experimental values were generally within 0.5 kcal mol(-1), with a very few exceptions.. Generally, steric interactions as well as intramolecular hydrogen bonding involving the dissociating OH group should be minimized in the GS. The DS are both electronically and vibrationally exited transition states. They have one unpaired electron on the carbon atom, which bears the homolytically dissociating OH group and are second order saddle points with a fixed

Topics: Algorithms; Antioxidants; Ascorbic Acid; Flavonoids; Gases; Hydroxides; Isomerism; Models, Theoretical; Molecular Structure; Phenols; Quantum Theory; Thermodynamics

This study presents the results of antioxidative and pro-oxidative efficacy of cysteamine and D-penicillamine (D-pen) in comparison to L-glutathione (L-GSH) on high-molar-mass hyaluronan (HA) degradation by cupric ions plus ascorbic acid.. The substance tested was applied in the degradative system cupric ions plus ascorbate: (i) before the reaction onset or also (ii) 1 h after the reaction started. The results obtained were compared with that one recorded by using the degradative system in the absence of the substance tested. To monitor HA degradation kinetics, rotational viscometry was applied. Moreover, the standard ABTS and DPPH assays were used.. By using the method of rotational viscometry, D-pen showed dual effect: initial inhibitory effect on •OH radicals was changed to a pro-oxidative one in the dose and time dependent manner. Both L-GSH and cysteamine were recorded to be more effective scavengers of •OH radicals than D-pen. Cysteamine demonstrated to be an excellent scavenger also of alkoxyl- and peroxyl- type radicals. Based on IC50 values, gained by ABTS assay, it is evident that D-pen showed higher radical scavenging capacity compared to cysteamine. Similar results were observed also in DPPH assay, although in this assay less effective radical scavenging capacities of both substances tested were recorded.. On the basis of the results obtained, it can be stated that D-pen can produce hydrogen peroxide or •OH radicals and can inhibit the production of these oxidants. Our results showed that both L-GSH and cysteamine are similarly effective in inhibiting of HA degradation. Moreover, cysteamine demonstrated to be a significant inhibitor of alkoxyl- and peroxyl- type radicals generated from C-type macroradical of HA.

Topics: Antioxidants; Ascorbic Acid; Copper; Cysteamine; Dose-Response Relationship, Drug; Glutathione; Hyaluronic Acid; Hydrogen Peroxide; Hydroxides; Oxidation-Reduction; Penicillamine; Reactive Oxygen Species; Sulfhydryl Compounds; Time Factors

This study was aimed to evaluate the oxidative damage, production of reactive oxygen species and the status of antioxidative defenses following cerebral GSH depletion induced by two classical depletors, diethylmaleate (DEM, 3 mmol/kg, i.p.) and phorone (PHO, 4 mmol/kg, i.p.). The treatment decreased (40-43%) brain glutathione levels at 2 h, followed by a partial recovery at 24 h. Cerebral glutathione depletion by these agents increased the levels of superoxide anion and hydroxyl radical at both the time intervals; however, hydrogen peroxide was high at 24 h only. It also produced a dramatic increase in the protein carbonyls at 2 h but not at 24h, without any significant effect on lipid peroxidation and conjugated diene levels. These rats showed a significantly lowered superoxide dismutase activity both at 2 h and 24 h of exposure, as compared to controls. Glutathione depletion enhanced catalase activity markedly at 2 h, followed by some recovery at 24 h. While Se-independent glutathione peroxidase (GPx) and glutathione S-transferase activities were increased at both 2 and 24 h time intervals, Se-dependent GPx and glucose-6-phosphate dehydrogenase were induced at 2 h only. Glutathione depletion decreased ceruloplasmin and vitamin E levels significantly at 2 h. However, ascorbic acid remained unaffected. It may be concluded that an acute cerebral glutathione depletion generates higher levels of reactive oxygen species, which may be responsible for oxidative modification of proteins. Some of these changes appear to recover soon after an activation of a variety of cellular antioxidant defense mechanisms and glutathione restoration. It appears that central nervous system is highly vulnerable to oxidative damage following a moderate glutathione depletion that may result from certain diseases or xenobiotic exposures.

Topics: Animals; Antioxidants; Ascorbic Acid; Brain; Ceruloplasmin; Free Radicals; gamma-Glutamyltransferase; Glutathione; Hydrogen Peroxide; Hydroxides; In Vitro Techniques; Ketones; Male; Maleates; Rats; Rats, Inbred Strains; Reactive Oxygen Species; Superoxides; Thiobarbituric Acid Reactive Substances; Vitamin E

We measured ascorbate radical (AR) produced by the reaction of ascorbic acid (Asc) with hydroxyl radical (.OH) or superoxide (O2-) after irradiation in normal muscle and SCC-VII tumor of C3H/He mice. AR can be measured using electron spin resonance (ESR) equipment and the dialysis method. The tube for collecting AR was inserted such that the dialysis membrane was in contact with the normal thigh or the center of the tumor lesion. The AR in the interstitial fluid around the membrane was collected through the dialysis membrane. After irradiation with 10 Gy, AR increased in both the normal muscle and tumor tissue; the percent increase was 53.1% for normal muscle tissue and 33.8% for tumor tissue. The maximum percent increases in AR in the normal muscle and the tumor tissue were 11.7 and 9.5% for 2.5 Gy, 28.5 and 18.5% for 5 Gy, 53.1 and 33.8% for 10 Gy, and 88.5 and 44.8% for 15 Gy, respectively. The amount of AR increased to maximums of 144.3% and 160.1% after treatment with H2O2 and FeCl2, respectively, while it decreased to minimums of 65.3% and 81.3% after treatment with superoxide dismutase (SOD) and catalase, respectively. These results suggest that the amount of .OH and O2- is reflected in the amount of AR production. This method is useful for the following reasons. First, no special treatment, such as freezing of the samples, and no administration of noxious agents are necessary. Second, irradiation using a dose of only several Gy shows an increase in the production of AR. Third, this method is less invasive.(ABSTRACT TRUNCATED AT 250 WORDS)

Topics: Animals; Ascorbic Acid; Carcinoma, Squamous Cell; Dose-Response Relationship, Radiation; Electron Spin Resonance Spectroscopy; Free Radicals; Hydroxides; Male; Mice; Mice, Inbred C3H; Radiation Dosage; Superoxides

Measurements of the rates for formation of conjugated dienes, malonylaldehyde, and lipid hydroperoxides show that increasing the concentration of O2 from 0.11 mM to 0.35 mM or 0.69 mM can slow the rate of linoleic acid peroxidation in a xanthine oxidase/hypoxanthine system. This effect is seen at pH 7.0 but not 7.4 and depends on the presence of monounsaturated fatty acids (oleic, cis, or trans vaccenic acid). Oxygen antagonism of ascorbic acid-iron-EDTA mediated lipid peroxidation is similarly dependent on fatty acid mixtures and occurs at pH 5.0 and 6.0 but not 7.0. The efficiency of initiation of peroxidation in the xanthine oxidase system is unaffected by monounsaturated fatty acids and O2 concentration. Increasing the O2 concentration increases the rate of superoxide radical production, but there is no change in salicylate hydroxylation (e.g., OH. production) or ferrous ion concentration. Oxygen-mediated slower rates of lipid peroxidation are associated with either increased H2O2 production or, based on an indirect assay, singlet O2 production. Increased O2 concentrations increase the rate of azobisisobutyronitrile-initiated lipid peroxidation as expected but addition of exogenous superoxide radicals slows the rate. Under similar conditions superoxide reacts with fatty acids to produce singlet O2. Overall, the data suggest that O2-mediated antagonism occurs because of termination reactions between hydroperoxyl (HO2.) and organic radicals, and singlet O2 or H2O2 are products of these reactions.

Topics: Ascorbic Acid; Bilirubin; Edetic Acid; Ferrous Compounds; Free Radicals; Hydrogen Peroxide; Hydroxides; Hypoxanthine; Hypoxanthines; Lipid Peroxidation; Oxidation-Reduction; Oxygen; Singlet Oxygen; Superoxides; Xanthine Oxidase