ascorbic-acid has been researched along with hippuryl-glycyl-glycine* in 2 studies
2 other study(ies) available for ascorbic-acid and hippuryl-glycyl-glycine
| Article | Year |
|---|---|
A novel multiple signal amplifying immunosensor based on the strategy of in situ-produced electroactive substance by ALP and carbon-based Ag-Au bimetallic as the catalyst and signal enhancer.
Topics: Alkaline Phosphatase; Animals; Ascorbic Acid; Biosensing Techniques; Carbon; Catalysis; Goats; Gold; Humans; Immunoconjugates; Immunoenzyme Techniques; Limit of Detection; Metal Nanoparticles; Nanotubes, Carbon; Oligopeptides; Silver | 2017 |
N-[3H]Benzoylglycylglycylglycine as a probe for hydroxyl radicals.
N-[4-(3)H]Benzoylglycylglycylglycine ([(3)H]BzG(3)) was tested as a probe for detecting hydroxyl radicals (*OH). Aerated solutions of l-ascorbate generated *OH, which oxidized [(3)H]BzG(3), yielding hydrophilic (probably hydroxylated) derivatives plus tritiated water. The (3)H(2)O was separated from organic products and remaining [(3)H]BzG(3) on Dowex-1. (3)H(2)O production was much greater with *OH than with other reactive oxygen species (ROS) (e.g., H(2)O(2), superoxide). The slight (3)H(2)O production in the presence of H(2)O(2) or superoxide was blocked by *OH scavengers (e.g., glycerol, mannitol, butan-1-ol) that do not scavenge H(2)O(2) or superoxide. This indicates that (3)H(2)O production was caused by *OH and that other ROS only generated any (3)H(2)O by forming traces of *OH. Doses of *OH that caused detectable nonenzymic polysaccharide scission also caused (3)H(2)O production, indicating that [(3)H]BzG(3) is a sensitive *OH probe in studies of polymer scission. The ability of scavengers and chelators to protect against ascorbate-mediated polysaccharide scission paralleled their ability to inhibit concurrent (3)H(2)O production, indicating that both processes were due to *OH. Thus, [(3)H]BzG(3) is a simple, specific, sensitive, and robust probe for detecting *OH production in vitro. It may have applications for in vivo detection of extracellular *OH in arthritic joints and of apoplastic *OH in plant cell walls. Topics: Ascorbic Acid; Chelating Agents; Free Radical Scavengers; Hydroxyl Radical; Oligopeptides; Oxidation-Reduction; Polysaccharides; Reactive Oxygen Species; Water | 2004 |