ascorbic-acid and fucoxanthin

Neutrophils provide the first line of defense of the innate immune system by phagocytosing, killing and digesting bacteria and fungi. During this process, neutrophils produce reactive oxygen species (ROS), which in excess, can damage the cells themselves and surrounding tissues. The carotenoid fucoxanthin (Fc) has been studied concerning its antioxidant and anti-inflammatory actions. Vitamin c (Vc) also demonstrates potent antioxidant action. This study aimed to evaluate the effect of Fc (2 μM) in association with Vc (100 μM) on functional parameters of human neutrophils in vitro.. We evaluated the migration and phagocytic capacity, intracellular calcium mobilization, ROS production (O₂(·)⁻, H₂O₂, HOCl), myeloperoxidase activity, profile of antioxidant enzymes, phosphorylation of p38 MAPK and p65 NFκB subunit, GSH/GSSG ratio and release of pro-inflammatory cytokines (TNF-α and IL-6) in neutrophils under different stimuli.. We verified an increase in phagocytic capacity for all treatments, together with an increase in intracellular calcium only in cells treated with Fc and Fc + Vc. ROS production was reduced by all treatments, although Vc was a better antioxidant than Fc. Phosphorylation of the p-65 subunit of NFκB was reduced in cells treated with Fc + Vc and release of TNF-α and IL-6 was reduced by all treatments. These findings indicate that the regulation of inflammatory cytokines by neutrophils is not exclusively under the control of the NFκB pathway. Fc reduced the activity of some antioxidant enzymes, whereas Vc increased GR activity and the GSH/GSSG ratio.. In conclusion, the results presented in this study clearly show an immunomodulatory effect of the carotenoid fc alone or in combination with Vc on the function of human neutrophils.

Topics: Adult; Anti-Inflammatory Agents, Non-Steroidal; Antioxidants; Ascorbic Acid; Calcium Signaling; Cell Movement; Cells, Cultured; Dietary Supplements; Female; Humans; Male; Neutrophil Activation; Neutrophils; Phagocytosis; Phosphorylation; Protein Processing, Post-Translational; Reactive Oxygen Species; Transcription Factor RelA; Xanthophylls; Young Adult

The aim of this study was to evaluate the effects of FUCO alone or combined with vitamin C on different features of lymphocyte function related to ROS/RNS (reactive oxygen/nitrogen species) production. For this purpose we have evaluated the cytotoxicity of increasing concentrations of FUCO and vitamin C, the proliferative capacity of stimulated T- and B-lymphocytes, superoxide anion radicals (O(2)), hydrogen peroxide (H(2)O(2)) and nitric oxide (NO) production, antioxidant enzyme activities and the indexes of oxidative damage in proteins (carbonyl and thiol content). We have also evaluated the release of inflammatory cytokines and glucose-6-phosphate dehydrogenase (G6PDH) activity. Healthy human lymphocytes were acutely treated in vitro with FUCO (2 μM) with or without vitamin C (100 μM). Results revealed that human lymphocytes treated with FUCO at 2μM did not present any significant alteration in the proliferation of T- and B-lymphocytes at both resting and stimulated conditions. Moreover, FUCO used at low concentrations showed more pro-oxidant than antioxidant effects, which were recognized by the increased H(2)O(2) and increased NO production. Anti-inflammatory activity of FUCO was confirmed by significantly increased IL-10 and decreased TNF-α production. Vitamin C increased T-lymphocyte proliferation, whereas vitamin C plus FUCO promoted a reduction in the proliferation rate of these cells. All groups decreased pro-inflammatory cytokine TNF-α and increased anti-inflammatory IL-10 production although only vitamin C decreased IFN-γ either alone or when combined with FUCO. Overall, the combination of the antioxidants had more antioxidant and anti-inflammatory effects than when they were applied alone.

Topics: Adolescent; Adult; Anti-Inflammatory Agents; Antioxidants; Ascorbic Acid; B-Lymphocytes; Cell Proliferation; Cells, Cultured; Cytokines; Drug Combinations; Drug Synergism; Female; Glucosephosphate Dehydrogenase; Humans; Inflammation Mediators; Lymphocyte Activation; Male; Nitric Oxide; Oxidative Stress; Protein Carbonylation; Reactive Oxygen Species; T-Lymphocytes; Xanthophylls; Young Adult