ascorbic-acid and estradiol-dipropionate

CBA female mice treated with 1,2-dimethylhydrazine (DMH) alone or in combination with oestradiol dipropionate (EP) or ascorbic acid (AA) developed, as expected, a high incidence of uterine sarcomas. In addition, sarcomatous lesions at unusual sites (mainly in the forestomach) were evident. The incidence of sarcomatous lesions at other sites was 53/220 in mice having uterine sarcomas and 0/186 in mice treated with DMH but without uterine sarcomas. The difference between the two groups was highly statistically significant (P < 0.001) and demonstrates non-coincidental association of the above sarcomatous lesions with uterine sarcomas. Uterine sarcomas which presented in association with lesions at other sites were of a larger size than those found in isolation, and the difference in weights in three out of four groups was statistically significant (P = 0.008, 0.035 and 0.011). Histologically, sarcomatous lesions were similar in structure to those of uterine sarcomas, i.e. were of a fibroblastic-histiocytic nature with admixture of giant cells. On the basis of the above data the sarcomatous lesions described appear to represent uterine sarcoma metastases rather than independent primary tumours. AA did not have any influence on carcinogenesis induced by DMH alone but inhibited the growth of uterine sarcomas (whether or not they were associated with other sarcomatous lesions) induced by DMH combined with oestradiol dipropionate.

Topics: 1,2-Dimethylhydrazine; Animals; Ascorbic Acid; Carcinogens; Colonic Neoplasms; Dimethylhydrazines; Estradiol; Female; Incidence; Mice; Mice, Inbred CBA; Mutagens; Sarcoma; Stomach Neoplasms; Uterine Neoplasms

Topics: Animals; Ascorbic Acid; Carcinogens; Dimethylhydrazines; Estradiol; Female; Mice; Mice, Inbred CBA; Neoplasm Metastasis; Sarcoma, Experimental; Uterine Neoplasms

Administration of estradiol-dipropionate (EP) after the cessation of 1,2-dimethylhydrazine (DMH) treatment increased the incidence of uterine sarcomas in CBA mice from 32.5 (DMH alone) to 62.5%. Ascorbic acid (AA) (0.3% in drinking water) given simultaneously with EP decreased the tumour incidence to 35%. Sodium ascorbate did not exert an inhibiting effect. AA inhibited the increase of uterine weight produced in mice by EP and did not influence the growth of mouse transplantable uterine sarcomas. The mechanisms of the antiestrogenic effects of AA are discussed.

Topics: 1,2-Dimethylhydrazine; Animals; Ascorbic Acid; Carcinogens; Dimethylhydrazines; Drug Antagonism; Estradiol; Female; Life Tables; Mice; Mice, Inbred CBA; Organ Size; Sarcoma, Experimental; Uterine Neoplasms

The administration of estradiol propionate after the discontinuation of 1,2-dimethylhydrazine (DMH) treatment increased the incidence of uterine sarcoma in CBA mice from 32.5 (DMH alone) to 62.5%. The addition of the ascorbic acid (0.3% solution in drinking water) to estradiol propionate was followed by a decrease in the tumor incidence to 35%, i. e. the acid levelled the promoting effect of estradiol propionate almost completely. Sodium ascorbate did not share that effect. Mechanisms underlying the antiestrogenic effect of the ascorbic acid are discussed.

Topics: 1,2-Dimethylhydrazine; Animals; Ascorbic Acid; Carcinogens; Dimethylhydrazines; Drug Interactions; Drug Screening Assays, Antitumor; Estradiol; Estrogen Antagonists; Female; Mice; Mice, Inbred CBA; Sarcoma, Experimental; Uterine Neoplasms

Administration of ascorbic acid (0.3, 0.75 or 1.5%) in drinking water started after the treatment of female CBA mice with 1,2-dimethylhydrazine and estradiol-dipropionate inhibited the growth of uterine sarcomas. The inhibitory effect depended upon dosage to some extent. When administered together with estradiol-dipropionate to intact mice, ascorbic acid arrested uterine growth associated with estrogen treatment.

Topics: 1,2-Dimethylhydrazine; Animals; Ascorbic Acid; Body Weight; Dimethylhydrazines; Estradiol; Estrogen Antagonists; Female; Mice; Mice, Inbred CBA; Organ Size; Sarcoma, Experimental; Uterine Neoplasms; Uterus

The purpose of this study was to determine if plasma mineral levels and plasma or hepatic ascorbic acid would be affected by the composition of the diet fed to chickens with higher circulating estrogen levels. Three-week-old broiler chicks were implanted with estradiol dipropionate to give estimated release rates of 0, 3.2, and 8.0 micrograms/bird/day. The chicks were fed a corn-soy diet (CS) or a diet containing 5% fish meal, 5% alfalfa meal and 10% torula yeast (FAY). The FAY diet resulted in significantly lower hepatic lipid and plasma iron, copper, manganese, and zinc in chicks administered the highest level of estrogen. In two experiments laying hens were fed either CS or diets containing fish meal (FM), alfalfa meal (AM), distillers dried grains with solubles (DDGS) or wheat-soy (WS) for 4 weeks. In the first experiment, liver lipid content was not significantly affected by diet composition, but plasma iron was significantly reduced by the AM, DDGS, and WS diets. No significant effects on copper, manganese, and zinc in plasma were observed among the dietary treatments. In the second experiment, relative liver weight was significantly reduced with the AM, DDGS, and WS diets and liver lipid by the AM diet. Plasma iron levels were significantly reduced by feeding all diets compared with the CS diet, but no significant differences in total iron binding capacity were observed. Plasma and hepatic ascorbic acid were significantly increased by the AM, DDGS, and WS diets, but no significant differences in hepatic ascorbic acid were observed when calculated per unit of fat-free dry matter.(ABSTRACT TRUNCATED AT 250 WORDS)

Topics: Animal Feed; Animals; Ascorbic Acid; Body Weight; Chickens; Copper; Drug Implants; Estradiol; Female; Iron; Lipid Metabolism; Liver; Male; Manganese; Minerals; Organ Size; Oviposition; Zinc