ascorbic-acid and diphenylditelluride

The present study evaluated the effect of acute exposure to diphenyl ditelluride [(PhTe)(2)] on oxidative status in lungs of rats. Rats were exposed to a single subcutaneous application of (PhTe)(2) at the doses of 0.3, 0.6, 0.9 μmol/kg or vehicle. After 72 h of exposure to (PhTe)(2), biochemical parameters of oxidative stress were carried out in lungs of rats. The lungs of rats exposed to (PhTe)(2) showed an increase in the levels of lipid peroxidation, reactive species and non-protein thiol. Alterations in superoxide dismutase activity were observed at all tested doses. (PhTe)(2) caused an increase in catalase activity and a reduction in ascorbic acid levels at the dose of 0.9 μmol/kg. The oxidative damage was more pronounced in animals treated with the highest dose of (PhTe)(2). Thus, this study demonstrated that acute exposure to (PhTe)(2) induced oxidative damage and an adaptive response of antioxidants in pulmonary tissue of rats.

Topics: Animals; Ascorbic Acid; Benzene Derivatives; Catalase; Dose-Response Relationship, Drug; Female; Lung; Organometallic Compounds; Oxidative Stress; Porphobilinogen Synthase; Rats; Rats, Wistar; Reactive Oxygen Species; Sulfhydryl Compounds; Superoxide Dismutase; Thiobarbituric Acid Reactive Substances; Toxicity Tests, Acute

The present study was conducted to evaluate the toxicity of the exposure to diphenyl diselenide [(PhSe)2] and diphenyl ditelluride [(PhTe)2] on reproductive system in Wistar rats. Adult male rats were exposed intraperitonealy (acute) or subcutaneously (sub-chronic, during 4 or 8 weeks) to (PhSe)2 or (PhTe)2 prior to mating. A number of biochemical parameters in rat testes were examined, such as delta-aminolevulinate dehydratase (delta-ALA-D) activity, lipid peroxidation, glycogen content and components of the antioxidant defenses (superoxide dismutase (SOD) activity and ascorbic acid concentration). Furthermore, a possible effect on fertility and reproductive performance in male rats were studied. Sperm counts of caudal epididymis were also evaluated. No lethality was noted in any group. Reduction on body weight in rats which received (PhTe)2 was only evidenced in acute exposure, while (PhSe)2-exposed rats presented significant loss of body weight in acute and 4 week-exposure. Mating and fertility indexes were not affected after acute and sub-chronic exposure. Regarding other parameters studied, except for a decrease in testes glycogen content in acutely (PhSe)2-treated group, no alterations were found in treated groups. Sperm counts of rats treated acutely and sub-chronically were unaffected by drugs exposure. Histological evaluation revealed no modification on testicular tissue in rats exposed to (PhSe)2 and (PhTe)2. The results suggest the absence of the male reproductive toxicity induced by (PhSe)2 and (PhTe)2 administered intraperitonealy (acute) or subcutaneously (sub-chronical) to adult rats Wistar.

Topics: Animals; Ascorbic Acid; Benzene Derivatives; Body Weight; Female; Fertility; Glycogen; Injections, Intraperitoneal; Injections, Subcutaneous; Lipid Peroxidation; Male; Organometallic Compounds; Organoselenium Compounds; Porphobilinogen Synthase; Pregnancy; Rats; Rats, Wistar; Reproduction; Sperm Count; Superoxide Dismutase; Testis; Thiobarbituric Acid Reactive Substances; Toxicity Tests, Acute; Toxicity Tests, Chronic