ascorbic-acid and cobalt-phthalocyanine

Topics: Ascorbic Acid; Biosensing Techniques; Carbon; Electrochemical Techniques; Electrochemistry; Electrodes; Flow Injection Analysis; Hydrogen Peroxide; Indoles; Kinetics; Limit of Detection; Meat; Myoglobin; Organometallic Compounds; Peroxynitrous Acid; Plant Extracts

In the present work, ternary mixtures of Acetaminophen, Ascorbic acid and Uric acid were resolved using the Electronic tongue (ET) principle and Cyclic voltammetry (CV) technique. The screen-printed integrated electrode array having differentiated response for the three oxidizable compounds was formed by Graphite, Prussian blue (PB), Cobalt (II) phthalocyanine (CoPc) and Copper oxide (II) (CuO) ink-modified carbon electrodes. A set of samples, ranging from 0 to 500 µmol·L

Topics: Acetaminophen; Ascorbic Acid; Biosensing Techniques; Copper; Electrochemical Techniques; Electrodes; Electronic Nose; Ferrocyanides; Graphite; Humans; Indoles; Organometallic Compounds; Uric Acid

High-molecular-weight DNA fragments are the markers of the early stage of apoptosis induced in eukaryotic cells by cytotoxins of different nature. The dynamics of the development of large-scale DNA fragmentation in K-562 leukemia cells by the action of the antitumor drug, the binary system ascorbic acid--cobalt phthalocyanine within 48 h of incubation, which correspond to two periods of the doubling of cell number in growing control cultures, have been studied. It was shown that, within the first hours of incubation, hydrogen peroxide generated by the system induces the formation of DNA fragments from 2200 to 50 kbp long. Later on the cell death accompanied by a decrease in the content of fragmented DNA is observed. Within 24 h of incubation, part of fragmented DNA remains unrepaired; after 48 h of incubation, a delay or a slowed down proliferation of K-562 cells, which differ from control cells also by a high level of death and a higher content of high-molecular-weight DNA fragments, is observed.

Topics: Antineoplastic Combined Chemotherapy Protocols; Ascorbic Acid; Cell Death; Cell Division; Cell Proliferation; DNA; DNA Fragmentation; Electrophoresis, Gel, Pulsed-Field; Humans; Hydrogen Peroxide; Indoles; K562 Cells; Molecular Weight; Organometallic Compounds

It was demonstrated that ascorbate-cobalt phthalocyanine complex produces a time-dependent nuclease effect on leukemia K-562 cells is. Catalase added to the incubation medium prevented or blocked fragmentation of cell DNA. The size of large-scale fragments formed during irradiation and exposure to the above system varied from 2200 to 30 kbp. The fragments induced by the system recombined slower than the fragments induced by g-irradiation in a dose adequate by the level of DNA damage. This effect observed previously in HEp-2 carcinoma cells exposed to the action of the B12b+C vitamin system can be explained by generation of H(2)O(2) inducing more severe damage to DNA structure than gamma-radiation due to site-specific Fenton reaction.

Topics: Apoptosis; Ascorbic Acid; DNA Fragmentation; DNA Repair; DNA, Neoplasm; Gamma Rays; Humans; Indoles; K562 Cells; Organometallic Compounds; Reactive Oxygen Species

Conjugates of cobalt and aluminum phthalocyanines with monoclonal antibodies were synthesized using an AOT/n-octane reversed micellar system or water-organic mixtures with a low content of organic solvent as media. The effect of the degree of hydration of the micelles and the concentration of phthalocyanines on the composition of conjugates was studied. The immune activity of the resulting conjugates in comparison to that of native antibodies was evaluated. The catalytic activity of free cobalt phthalocyanines and their antibody conjugates was studied in the reaction of ascorbic acid oxidation.

Topics: Aluminum; Antibodies, Monoclonal; Ascorbic Acid; Cobalt; Dioctyl Sulfosuccinic Acid; Immunoconjugates; Indoles; Micelles; Octanes; Organometallic Compounds; Ovalbumin; Oxidation-Reduction; Solvents; Spectrometry, Fluorescence; Water