ascorbic-acid has been researched along with bacteriopheophytin* in 2 studies
2 other study(ies) available for ascorbic-acid and bacteriopheophytin
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Reconstitution and replacement of bacteriochlorophyll a molecules in photosynthetic reaction centers.
Reaction centers (RCs) of the photosynthetic bacterium Rhodobacter sphaeroides R-26 were reconstituted in liposomes after release of pigments (bacteriochlorophyll a (BChla) and bacteriopheophytin a (BPhea)) by treatment with acetone. As shown by absorption and circular dichroism spectroscopies, the reconstituted RCs had the same arrangement of pigments as the native RC and exhibited photoactivity of the special pair. The recovery yield of RCs of up to 30% was achieved by addition of 7.8-fold excess of BChla in the acetone treatment. Furthermore BChla was partially replaced with Zn-BChla by addition of the pigments during the acetone treatment. About 30% and 50% of the special pair and accessory pigments can be replaced with Zn-BChla, respectively. From this rate, an oxidation-reduction potential of 520 mV (vs. the normal hydrogen electrode NHE) was derived by the simulation of the experimental data, which is 35 mV higher than that of the native RC (484 mV vs. NHE). Topics: Acetone; Ascorbic Acid; Bacteriochlorophyll A; Bacteriochlorophylls; Chromatography, High Pressure Liquid; Circular Dichroism; Electrodes; Hydrogen; Light; Light-Harvesting Protein Complexes; Liposomes; Oxidation-Reduction; Oxygen; Pheophytins; Photosynthetic Reaction Center Complex Proteins; Rhodobacter sphaeroides; Spectrophotometry; Time Factors; Zinc | 2004 |
Origin of optical activity in the purple bacterial photoreaction center.
The photoreaction center (RC) of purple bacteria contains four bacteriochlorophyll (Bch) and two bacteriopheophytin (Bph) molecules as prosthetic groups. Their optical activity, as measured by circular dichroism (CD) spectroscopy, is largely increased in situ as compared to organic solutions. The all-exciton hypothesis posits that this enhanced optical activity is entirely due to excitonic interactions between the electronic transitions of all six bacteriochlorin molecules. Using the simple exciton theory, this model predicts that the near-infrared CD spectra should be conservative. The fact that they are not, whether the special pair of Bch (SP) that constitutes the primary electron donor is reduced or oxidized, has been explained by hyperchromic effects. The present work tests this hypothesis by successively eliminating the absorption and, therefore, the optical activity of the Bphs and of the non-special-pair (non-SP) Bchs. This was accomplished by trapping these pigments in their reduced state. RC preparations with the four non-SP bacteriochlorins trapped in their reduced state and, therefore, with an intact SP displayed conservative CD spectra. RC preparations with only the electronic transitions of SP and of one non-SP Bch also showed conservative CD spectra. These conservative CD spectra and their corresponding absorption spectra were simulated using simple exciton theory without assuming hyperchromic effects. Bleaching half of the 755-nm absorption band by phototrapping one of the two Bph molecules led to the complete disappearnce of the corresponding CD band. This cannot be explained by the all-exciton hypothesis. These results suggest that the optical activity of the SP alone, or with one non-SP Bch, is due to excitonic interactions.(ABSTRACT TRUNCATED AT 250 WORDS) Topics: Ascorbic Acid; Bacteriochlorophylls; Chromatiaceae; Circular Dichroism; Dithionite; Electron Transport; Light-Harvesting Protein Complexes; Optics and Photonics; Oxidation-Reduction; Pheophytins; Photochemistry; Photosynthetic Reaction Center Complex Proteins; Spectrophotometry | 1995 |