ascorbic-acid and araldite

ascorbic-acid has been researched along with araldite* in 1 studies

Other Studies

1 other study(ies) available for ascorbic-acid and araldite

Article	Year
Construction of an amperometric ascorbate biosensor using epoxy resin membrane bound Lagenaria siceraria fruit ascorbate oxidase. Artificial cells, blood substitutes, and immobilization biotechnology, 2011, Volume: 39, Issue:3 Ascorbate oxidase purified from Lagenaria siceraria fruit was immobilized onto epoxy resin "Araldite" membrane with 79.4% retention of initial activity of free enzyme. The biosensor showed optimum response within 15s at pH 5.8 and 35°C, which was directly proportional to ascorbate concentration ranging from 1-100μM. There was a good correlation (R(2) = 0.99) between serum ascorbic acid values by standard enzymic colorimetric method and the present method. The enzyme electrode was used for 200 times without considerable loss of activity during the span of 90 days when stored at 4°C. Topics: Ascorbate Oxidase; Ascorbic Acid; Biosensing Techniques; Colorimetry; Cucurbitaceae; Enzymes, Immobilized; Epoxy Resins; Fruit; Humans; Phthalic Anhydrides; Plant Proteins	2011

Article

Year

Construction of an amperometric ascorbate biosensor using epoxy resin membrane bound Lagenaria siceraria fruit ascorbate oxidase.

Artificial cells, blood substitutes, and immobilization biotechnology, 2011, Volume: 39, Issue:3

Ascorbate oxidase purified from Lagenaria siceraria fruit was immobilized onto epoxy resin "Araldite" membrane with 79.4% retention of initial activity of free enzyme. The biosensor showed optimum response within 15s at pH 5.8 and 35°C, which was directly proportional to ascorbate concentration ranging from 1-100μM. There was a good correlation (R(2) = 0.99) between serum ascorbic acid values by standard enzymic colorimetric method and the present method. The enzyme electrode was used for 200 times without considerable loss of activity during the span of 90 days when stored at 4°C.

Topics: Ascorbate Oxidase; Ascorbic Acid; Biosensing Techniques; Colorimetry; Cucurbitaceae; Enzymes, Immobilized; Epoxy Resins; Fruit; Humans; Phthalic Anhydrides; Plant Proteins

2011