ascorbic-acid and apramycin

ascorbic-acid has been researched along with apramycin* in 1 studies

Other Studies

1 other study(ies) available for ascorbic-acid and apramycin

Article	Year
Structural features and oxidative stress towards plasmid DNA of apramycin copper complex. Dalton transactions (Cambridge, England : 2003), 2009, Feb-21, Issue:7 The interaction of apramycin with copper at different pH values was investigated by potentiometric titrations and EPR, UV-vis and CD spectroscopic techniques. The Cu(II)-apramycin complex prevailing at pH 6.5 was further characterized by NMR spectroscopy. Metal-proton distances derived from paramagnetic relaxation enhancements were used as restraints in a conformational search procedure in order to define the structure of the complex. Longitudinal relaxation rates were measured with the IR-COSY pulse sequence, thus solving the problems due to signal overlap. At pH 6.5 apramycin binds copper(II) with a 2 : 1 stoichiometry, through the vicinal hydroxyl and deprotonated amino groups of ring III. Plasmid DNA electrophoresis showed that the Cu(II)-apramycin complex is more active than free Cu(II) in generating strand breakages. Interestingly, this complex in the presence of ascorbic acid damages DNA with a higher yield than in the presence of H(2)O(2). Topics: Antioxidants; Ascorbic Acid; Circular Dichroism; Copper; DNA; Electron Spin Resonance Spectroscopy; Hydrogen Peroxide; Hydrogen-Ion Concentration; Magnetic Resonance Spectroscopy; Models, Chemical; Nebramycin; Oxidants; Oxidative Stress; Plasmids; Thermodynamics	2009

Article

Year

Structural features and oxidative stress towards plasmid DNA of apramycin copper complex.

Dalton transactions (Cambridge, England : 2003), 2009, Feb-21, Issue:7

The interaction of apramycin with copper at different pH values was investigated by potentiometric titrations and EPR, UV-vis and CD spectroscopic techniques. The Cu(II)-apramycin complex prevailing at pH 6.5 was further characterized by NMR spectroscopy. Metal-proton distances derived from paramagnetic relaxation enhancements were used as restraints in a conformational search procedure in order to define the structure of the complex. Longitudinal relaxation rates were measured with the IR-COSY pulse sequence, thus solving the problems due to signal overlap. At pH 6.5 apramycin binds copper(II) with a 2 : 1 stoichiometry, through the vicinal hydroxyl and deprotonated amino groups of ring III. Plasmid DNA electrophoresis showed that the Cu(II)-apramycin complex is more active than free Cu(II) in generating strand breakages. Interestingly, this complex in the presence of ascorbic acid damages DNA with a higher yield than in the presence of H(2)O(2).

Topics: Antioxidants; Ascorbic Acid; Circular Dichroism; Copper; DNA; Electron Spin Resonance Spectroscopy; Hydrogen Peroxide; Hydrogen-Ion Concentration; Magnetic Resonance Spectroscopy; Models, Chemical; Nebramycin; Oxidants; Oxidative Stress; Plasmids; Thermodynamics

2009