ascorbic-acid and ammonium-ferric-sulfate

ascorbic-acid has been researched along with ammonium-ferric-sulfate* in 3 studies

Other Studies

3 other study(ies) available for ascorbic-acid and ammonium-ferric-sulfate

ArticleYear
Mefloquine in combination with hemin causes severe damage to adult Schistosoma japonicum in vitro.
    Acta tropica, 2014, Volume: 131

    In order to explore the interaction of mefloquine with hemin against adult Schistosoma japonicum in vitro, the 50% and 95% lethal concentration (LC50 and LC95) of mefloquine and hemin against schistosomes, some factors, such as other iron providing agents, iron chelaters, zinc protoporphyrin-IX, and biological relevant reductants, that might impact on antischistosomal activity induced by interaction of mefloquine with hemin, and preliminary analysis of chemical interaction of both compounds were undertaken. The LC50 and LC95 of mefloquine and hemin alone against schistosomes were determined to be 6.5μg/ml and 7.8μg/ml as well as 232μg/ml and 355μg/ml, respectively. The LC50 and LC95 of mefloquine in the presence of hemin 100μg/ml was 0.24μg/ml and 0.59μg/ml, respectively. On the other hand the LC50 and LC95 of hemin in the presence of mefloquine 1μg/ml was 23.2μg/ml and 77.2μg/ml, respectively. Meanwhile, mefloquine/hemin combinations showed potential synergistic effects against adult S. japonicum, with combination index (CI) values <1. Apart from hemin, zinc protoporphyrin-IX, and other iron providing agents such as ferrous sulfate and ferriammonium sulfate combined with mefloquine exhibited no toxic effect against schistosomes. On the other hand, addition of iron chelators (deferiprone, desferrioxamine mesylate, or 2,2'-bipyridine) to the medium containing mefloquine-hemin resulted in no protective effect on the worms. Furthermore, biological reductants like glutathione, vitamine C or cysteine showed no apparent worm protection effect from toxic mefloquine-hemin even at higher concentrations (242.3-614.6μg/ml, i.e., 6.4-17.8-fold higher than the concentration of hemin). Chemical interaction of mefloquine with hemin was studied in 40% DMSO-Tris buffer solution. Both UV-Vis spectrum and mass spectrum demonstrated the strong interaction of mefloquine with hemin, which resulted in a reduction of hemin color and emergence of an adduct formed by mefloquine and hemin. The results confirm that mefloquine combined with hemin exhibits potential synergistic effect against adult S. japonicum in vitro.

    Topics: 2,2'-Dipyridyl; Animals; Anthelmintics; Ascorbic Acid; Cysteine; Deferiprone; Deferoxamine; Drug Synergism; Drug Therapy, Combination; Ferric Compounds; Ferrous Compounds; Glutathione; Hemin; Inhibitory Concentration 50; Iron Chelating Agents; Mefloquine; Protoporphyrins; Pyridones; Schistosoma japonicum

2014
L-ascorbic acid microencapsulated with polyacylglycerol monostearate for milk fortification.
    Bioscience, biotechnology, and biochemistry, 2004, Volume: 68, Issue:3

    Efficiency was examined of microencapsulating L-ascorbic acid by polyglycerol monostearate (PGMS), and changes in the chemical and sensorial aspects of L-ascorbic acid and/or iron-fortified milk during storage were evaluated. The selected core materials were ferric ammonium sulfate and L-ascorbic acid. The highest efficiency (94.2%) of microencapsulation was found with the ratio of 5:1 as the coating to core material. The release of ascorbic acid from the microcapsules increased sharply from 1.6 to 6.7% up to 5 d of storage. The TBA value was the lowest in the milk sample with added encapsulated iron and unencapsulated L-ascorbic acid up to 5 d of storage in comparison with the other treated samples. A sensory analysis showed that most aspects were not significantly different between the control and fortified samples encapsulated with ascorbic acid after 5 d of storage. The results indicate that L-ascorbic acid microencapsulated with PGMS can be applied to fortify milk and acceptable milk products can be prepared with microencapsulated L-ascorbic acid and iron.

    Topics: Animals; Ascorbic Acid; Capsules; Ferric Compounds; Food, Fortified; Glycerol; Milk; Monoglycerides; Stearates; Thiobarbituric Acid Reactive Substances

2004
Retention of iron by rat intestine in vivo as affected by dietary fiber, ascorbate and citrate.
    The Journal of nutrition, 1986, Volume: 116, Issue:6

    The effects of pH, ascorbate, citrate and dietary fiber on retention of ferrous and ferric iron by jejuno-ileal segments of rat intestine were examined in vivo. Iron was introduced in an isosmotic solution of sodium chloride and dextrose buffered by 2-[bis(2-hydroxyethyl)amino]ethanesulfonic acid (BES) and acetate. Stabilization of the iron solutions was aided by use of iron concentrations less than or equal to 1 microgram/ml injected into the intestine for 10-min periods. Iron retention was optimal over a broad pH range from 5 to 7.8. Inclusion of ascorbic acid in the solution injected (5, 25 or 75 micrograms/ml) did not increase retention of iron in either valence state. A low concentration of sodium citrate (2 mM) had no effect on iron retention, but increasing the concentration to 5 mM released iron from the mucosa. Maize and wheat fibers decreased the retention of ferrous iron by binding and by promoting autoxidation and formation of poorly soluble iron polymers. Bound ferrous iron was released completely at pH below 5. Retention of ferric iron was also lowered in the presence of fiber, presumably as a result of polymerization. Retention of iron by the rat in the absence of ligands was independent of valence state.

    Topics: Animals; Ascorbic Acid; Biological Availability; Citrates; Citric Acid; Dietary Fiber; Female; Ferric Compounds; Ferrous Compounds; Hydrogen-Ion Concentration; Ileum; Intestinal Mucosa; Intestines; Iron; Jejunum; Male; Quaternary Ammonium Compounds; Rats; Rats, Inbred Strains; Triticum; Zea mays

1986
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