ascorbic-acid and acetylacetone

ascorbic-acid has been researched along with acetylacetone* in 1 studies

Other Studies

1 other study(ies) available for ascorbic-acid and acetylacetone

Article	Year
Artifacts in the determination of microsomal xenobiotic N-demethylation in the presence of ascorbic acid tris buffer and Nash reagent. Xenobiotica; the fate of foreign compounds in biological systems, 1988, Volume: 18, Issue:5 1. During investigation of microsomal xenobiotic N-demethylation in the presence of ascorbic acid, large increases in apparent enzymic activity were observed. 2. Examination of incubation components indicated that a non-enzymic interaction between ascorbic acid and Tris buffer, in the presence of acetylacetone and ammonium acetate (Nash reagent), was occurring. 3. The resulting chromophore had an absorption maximum at 412 nm that coincided with the absorption for the chromophore resulting from the interaction of the Nash reagent with the product (formaldehyde) of the enzymic reaction. 4. Strict controls of ascorbic acid potential chemical interaction with incubation components are required in enzymic studies. Topics: Acetates; Animals; Ascorbic Acid; Chemical Phenomena; Chemistry; Ketones; Male; Microsomes; Mixed Function Oxygenases; Oxidoreductases, N-Demethylating; Pentanones; Rats; Rats, Inbred Strains; Tromethamine	1988

Article

Year

Artifacts in the determination of microsomal xenobiotic N-demethylation in the presence of ascorbic acid tris buffer and Nash reagent.

Xenobiotica; the fate of foreign compounds in biological systems, 1988, Volume: 18, Issue:5

1. During investigation of microsomal xenobiotic N-demethylation in the presence of ascorbic acid, large increases in apparent enzymic activity were observed. 2. Examination of incubation components indicated that a non-enzymic interaction between ascorbic acid and Tris buffer, in the presence of acetylacetone and ammonium acetate (Nash reagent), was occurring. 3. The resulting chromophore had an absorption maximum at 412 nm that coincided with the absorption for the chromophore resulting from the interaction of the Nash reagent with the product (formaldehyde) of the enzymic reaction. 4. Strict controls of ascorbic acid potential chemical interaction with incubation components are required in enzymic studies.

Topics: Acetates; Animals; Ascorbic Acid; Chemical Phenomena; Chemistry; Ketones; Male; Microsomes; Mixed Function Oxygenases; Oxidoreductases, N-Demethylating; Pentanones; Rats; Rats, Inbred Strains; Tromethamine

1988