ascorbic-acid and 2--deoxyguanosine-5--phosphate

ascorbic-acid has been researched along with 2--deoxyguanosine-5--phosphate* in 2 studies

Other Studies

2 other study(ies) available for ascorbic-acid and 2--deoxyguanosine-5--phosphate

Article	Year
Fe(II) phthalocyanine catalyzed oxidation of dGMP by molecular oxygen. Bioorganic & medicinal chemistry letters, 2009, Aug-01, Volume: 19, Issue:15 We show that iron(II)-phthalocyanines are able to catalyze guanosine oxidation by molecular oxygen in the presence of reducing agents such as ascorbic acid and 2-mercaptoethanol. The products of 5'-monophosphate-2'-deoxyguanosine (dGMP) oxidation were directly analyzed using the HPLC-ESI/MS method. The main oxidation products were 5'-phospho-2'-deoxy-8-oxo-7,8-dihydroguanine and the 1,N2-glyoxal adduct of the 5'-monophosphate-2'-deoxyguanosine. Topics: Antineoplastic Agents; Antiviral Agents; Ascorbic Acid; Catalysis; Chemistry, Pharmaceutical; Chromatography, High Pressure Liquid; Deoxyguanine Nucleotides; Drug Design; Humans; Hydroxyl Radical; Indoles; Iron; Isoindoles; Mercaptoethanol; Models, Chemical; Oxygen; Spectrometry, Mass, Electrospray Ionization	2009
Pulse radiolytic study of the interaction of thiols and ascorbate with OH adducts of dGMP and dG: implications for DNA repair processes. Radiation research, 1983, Volume: 96, Issue:1 Using the technique of pulse radiolysis, the interaction of .OH radicals with 2'-deoxyguanosine (dG) and dG-5'-monophosphate (dGMP) has been shown to result in the production of intermediates with different redox properties as demonstrated by their reactions with tetranitromethane (TNM) and N,N,N',N'-tetramethyl-p-phenylenediamine. The ratio of the yields of oxidizing to reducing-type .OH radical adducts of dGMP was determined to be about 1:1 and independent of pH (6-11). The nature of the intermediates produced on reaction of .OH with dGMP are discussed. The thiols, cysteine, glutathione, mercaptoacetic acid, and ascorbate have been shown to interact with those .OH adducts of dGMP and dG with oxidizing properties preferentially via an electron transfer process (k approximately 3 X 10(7)-1.4 X 10(9) dm3 mole-1 sec-1) as implied from the pH dependence of the rate constants. It is further demonstrated that oxygen and TNM do not interact with those .OH adducts of the purines with oxidizing properties. The implications of these findings are discussed with reference to the mechanistic aspects of radioprotection and especially of radiosensitization. Topics: Ascorbic Acid; Deoxyguanine Nucleotides; Deoxyguanosine; DNA Repair; Drug Interactions; Free Radicals; Hydrogen-Ion Concentration; Hydroxides; Oxidation-Reduction; Particle Accelerators; Pulse Radiolysis; Radiation Tolerance; Sulfhydryl Compounds	1983

Article

Year

Fe(II) phthalocyanine catalyzed oxidation of dGMP by molecular oxygen.

Bioorganic & medicinal chemistry letters, 2009, Aug-01, Volume: 19, Issue:15

We show that iron(II)-phthalocyanines are able to catalyze guanosine oxidation by molecular oxygen in the presence of reducing agents such as ascorbic acid and 2-mercaptoethanol. The products of 5'-monophosphate-2'-deoxyguanosine (dGMP) oxidation were directly analyzed using the HPLC-ESI/MS method. The main oxidation products were 5'-phospho-2'-deoxy-8-oxo-7,8-dihydroguanine and the 1,N2-glyoxal adduct of the 5'-monophosphate-2'-deoxyguanosine.

Topics: Antineoplastic Agents; Antiviral Agents; Ascorbic Acid; Catalysis; Chemistry, Pharmaceutical; Chromatography, High Pressure Liquid; Deoxyguanine Nucleotides; Drug Design; Humans; Hydroxyl Radical; Indoles; Iron; Isoindoles; Mercaptoethanol; Models, Chemical; Oxygen; Spectrometry, Mass, Electrospray Ionization

2009

Pulse radiolytic study of the interaction of thiols and ascorbate with OH adducts of dGMP and dG: implications for DNA repair processes.

Radiation research, 1983, Volume: 96, Issue:1

Using the technique of pulse radiolysis, the interaction of .OH radicals with 2'-deoxyguanosine (dG) and dG-5'-monophosphate (dGMP) has been shown to result in the production of intermediates with different redox properties as demonstrated by their reactions with tetranitromethane (TNM) and N,N,N',N'-tetramethyl-p-phenylenediamine. The ratio of the yields of oxidizing to reducing-type .OH radical adducts of dGMP was determined to be about 1:1 and independent of pH (6-11). The nature of the intermediates produced on reaction of .OH with dGMP are discussed. The thiols, cysteine, glutathione, mercaptoacetic acid, and ascorbate have been shown to interact with those .OH adducts of dGMP and dG with oxidizing properties preferentially via an electron transfer process (k approximately 3 X 10(7)-1.4 X 10(9) dm3 mole-1 sec-1) as implied from the pH dependence of the rate constants. It is further demonstrated that oxygen and TNM do not interact with those .OH adducts of the purines with oxidizing properties. The implications of these findings are discussed with reference to the mechanistic aspects of radioprotection and especially of radiosensitization.

Topics: Ascorbic Acid; Deoxyguanine Nucleotides; Deoxyguanosine; DNA Repair; Drug Interactions; Free Radicals; Hydrogen-Ion Concentration; Hydroxides; Oxidation-Reduction; Particle Accelerators; Pulse Radiolysis; Radiation Tolerance; Sulfhydryl Compounds

1983