ascorbic-acid has been researched along with 1-methyl-1-2-3-4-tetrahydro-beta-carboline-3-carboxylic-acid* in 2 studies
2 other study(ies) available for ascorbic-acid and 1-methyl-1-2-3-4-tetrahydro-beta-carboline-3-carboxylic-acid
| Article | Year |
|---|---|
Naturally-occurring tetrahydro-β-carboline alkaloids derived from tryptophan are oxidized to bioactive β-carboline alkaloids by heme peroxidases.
β-Carbolines are indole alkaloids that occur in plants, foods, and endogenously in mammals and humans, and which exhibit potent biological, psychopharmacological and toxicological activities. They form from naturally-occurring tetrahydro-β-carboline alkaloids arising from tryptophan by still unknown way and mechanism. Results in this research show that heme peroxidases catalyzed the oxidation of tetrahydro-β-carbolines (i.e. 1,2,3,4-tetrahydro-β-carboline-3-carboxylic acid and 1-methyl-1,2,3,4-tetrahydro-β-carboline-3-carboxylic acid) into aromatic β-carbolines (i.e. norharman and harman, respectively). This oxidation followed a typical catalytic cycle of peroxidases through redox intermediates I, II, and ferric enzyme. Both, plant peroxidases (horseradish peroxidase, HRP) and mammalian peroxidases (myeloperoxidase, MPO and lactoperoxidase, LPO) catalyzed the oxidation in an efficient manner as determined by kinetic parameters (VMAX and KM). Oxidation of tetrahydro-β-carbolines was inhibited by peroxidase inhibitors such as sodium azide, ascorbic acid, hydroxylamine and excess of H2O2. The formation of aromatic β-carbolines by heme peroxidases can help to explain the presence and activity of these compounds in biological systems. Topics: Ascorbic Acid; Carbolines; Enzyme Inhibitors; Harmine; Heme; Horseradish Peroxidase; Hydroxylamine; Kinetics; Lactoperoxidase; Oxidation-Reduction; Peroxidase; Peroxidases; Sodium Azide; Tryptophan | 2014 |
Antioxidants and cataract: (cataract induction in space environment and application to terrestrial aging cataract).
The effect of several antioxidants and cysteine-elevating precursor drugs (prodrugs) was tested on lens damage occurring after in vitro exposure to low levels of 60Co-gamma-irradiation, to simulate in vitro the exposure to radiation in vivo of (1) astronauts (2) jet crews (3) military radiation accident personnel. Tocopherol (100 microM), ascorbic acid (1 mM), R-alpha-lipoic acid (1 mM), and taurine (0.5 mM) protected against radiation-associated protein leakage. MTCA and ribocysteine protected lenses against opacification, LDH and protein leakage, indicating that antioxidants and prodrugs of cysteine appear to offer protection against lens damage caused by low level radiation. Topics: Animals; Antioxidants; Ascorbic Acid; Carbolines; Cataract; Cysteine; Dose-Response Relationship, Radiation; In Vitro Techniques; L-Lactate Dehydrogenase; Lens, Crystalline; Occupational Diseases; Radiation-Protective Agents; Rats; Ribose; Taurine; Thioctic Acid; Vitamin E | 1997 |