ascorbate-2-phosphate and 3-3--5-5--tetramethylbenzidine

A two-dimensional (2D) Co-MOF nanosheet-based nanozyme was developed for colorimetric detection of disease-related biomolecules. The prepared 2D Co-MOFs exhibited ultrahigh peroxidase catalytic activity. 2D Co-MOFs can catalyze the oxidation of colorless 3,3',5,5'-tetramethylbenzidine (TMB) to the blue product oxTMB, accompanying an obvious change of absorption value at 652 nm. However, alkaline phosphatase can catalyze the hydrolysis of L-ascorbic acid-2-phosphate to produce ascorbic acid which can reduce the oxTMB to TMB, resulting in an obvious color fading. Therefore, by recording the change of absorption value at 652 nm, the 2D Co-MOF nanosheets were used to detect ascorbic acid (AA) and alkaline phosphatase (ALP). The limit of detection for AA and ALP was 0.47 μM and 0.33 U L

Topics: Alkaline Phosphatase; Ascorbic Acid; Benzidines; Catalysis; Chromogenic Compounds; Cobalt; Colorimetry; Humans; Limit of Detection; Metal-Organic Frameworks; Nanostructures; Oxidation-Reduction; Paper; Smartphone

Topics: Acid Phosphatase; Ascorbic Acid; Benzidines; Chlorides; Chromogenic Compounds; Colorimetry; Fluorescent Dyes; Gold Compounds; Humans; Limit of Detection; Oxidation-Reduction; Rhodamines; Smartphone; Spectrometry, Fluorescence

Core-shell palladium cube@CeO

Topics: Alkaline Phosphatase; Ascorbic Acid; Benzidines; Catalysis; Cerium; Colorimetry; Coloring Agents; Enzyme Assays; Limit of Detection; Metal Nanoparticles; Oxidation-Reduction; Palladium