arthrofactin has been researched along with surfactin-peptide* in 4 studies
4 other study(ies) available for arthrofactin and surfactin-peptide
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A cyclic lipopeptide surfactin is a species-selective Hsp90 inhibitor that suppresses cyanobacterial growth.
Heat shock protein 90 (Hsp90) is essential for eukaryotic cells, whereas bacterial homologs play a role under stresses and in pathogenesis. Identifying species-specific Hsp90 inhibitors is challenging because Hsp90 is evolutionarily conserved. We found that a cyclic lipopeptide surfactin inhibits the ATPase activity of Hsp90 from the cyanobacterium Synechococcus elongatus (S.elongatus) PCC 7942 but does not inhibit Escherichia coli (E.coli), yeast and human Hsp90s. Molecular docking simulations indicated that surfactin could bind to the N-terminal dimerization interface of the cyanobacterial Hsp90 in the ATP- and ADP-bound states, which provided molecular insights into the species-selective inhibition. The data suggest that surfactin inhibits a rate-limiting conformational change of S.elongatus Hsp90 in the ATP hydrolysis. Surfactin also inhibited the interaction of the cyanobacterial Hsp90 with a model substrate, and suppressed S.elongatus growth under heat stress, but not that of E.coli. Surfactin did not show significant cellular toxicity towards mammalian cells. These results indicate that surfactin inhibits the cellular function of Hsp90 specifically in the cyanobacterium. The present study shows that a cyclic peptide has a great specificity to interact with a specific homolog of a highly conserved protein family. Topics: Adenosine Triphosphatases; Adenosine Triphosphate; Animals; Anti-Bacterial Agents; Chlorocebus aethiops; Colistin; COS Cells; Dimerization; Escherichia coli; HSP90 Heat-Shock Proteins; Humans; Hydrolysis; Lipopeptides; Mice; Molecular Docking Simulation; NIH 3T3 Cells; Peptides, Cyclic; Saccharomyces cerevisiae; Synechococcus | 2021 |
Novel characteristics of sophorolipids, yeast glycolipid biosurfactants, as biodegradable low-foaming surfactants.
Sophorolipids (SLs) are a family of glycolipid type biosurfactants, which are largely produced by the non-pathogenic yeast, Candida bombicola. In order to investigate the possibility of SLs for industrial use, here we examined the interfacial activities, cytotoxicity and biodegradability of SLs, and compared these properties with those of two lipopeptide type biosurfactants (surfactin and arthrofactin), sodium laurate (soap, SP) and four kinds of chemically synthesized surfactants including two block-copolymer nonionic surfactants (BPs), polyoxyethylene lauryl ether (AE) and sodium dodecyl sulfate (SDS). It was indicated that SLs had extremely low-foaming properties and high detergency comparable with commercially available low-foaming BPs. These interfacial activities of SLs were maintained under 100 ppm water hardness. Cytotoxicity of SLs on human keratinocytes was the same as surfactin, which has already been commercialized as cosmetic material, but higher than BPs. Moreover, biodegradability of SLs using the OECD Guidelines for Testing of Chemicals (301C, Modified MITI Test) displayed that SLs can be classified as "readily" biodegradable chemicals, which are defined as chemicals that are degraded 60% within 28 days under specified test methods. We observed 61% degradation of SLs on the eighth day of cultivation. Our results indicate that SLs are low-foaming surfactants with high detergency, which also exhibit both low cytotoxicity and readily biodegradable properties. Topics: Candida; Cells, Cultured; Glycolipids; Humans; Keratinocytes; Lipopeptides; Peptides, Cyclic; Polidocanol; Polyethylene Glycols; Sodium Dodecyl Sulfate; Surface-Active Agents | 2009 |
A study on the structure-function relationship of lipopeptide biosurfactants.
Arthrofactin (AF) and surfactin (SF) are the most effective cyclic lipopeptide biosurfactants ever reported. Linear AF and linear SF were prepared by saponification of lactone ring. The oil displacement activities decreased to one third of their respective original values. When residues of both an aspartic acid and a glutamic acid of SF were methylated or amidated, the activity increased by 20%, although their water solubility was lost. When these amino acid residues were modified by aminomethane sulfonic acid, the activity was drastically decreased probably owing to charge repulsion and structural distortion inhibiting micelle formation. Both AF and SF expressed higher activity under alkaline conditions than acidic conditions. AF was more resistant to acidic conditions than SF and it kept high activity even under pH 0.5. Although SF drastically reduced its activity under acidic conditions, surfactin-Asp/Glu-amido ester and surfactin-Asp/Glu-methyl ester retained similar activities irrespective of the pH change. A couple of conformers of SF prepared by reverse-phase HPLC showed the same oil displacement activity but different surface tension-reducing activity. AF was produced as a series of different fatty acid chain lengths (from C8 to C12). Among them, AF with fatty acid chain length of C10, which was the main product of the strain, showed the highest activity. Topics: Bacterial Proteins; Fatty Acids; Hydrogen-Ion Concentration; Lactones; Lipopeptides; Lipoproteins; Peptides, Cyclic; Protein Isoforms; Structure-Activity Relationship; Surface Tension; Surface-Active Agents | 2000 |
A new lipopeptide biosurfactant produced by Arthrobacter sp. strain MIS38.
A biosurfactant termed arthrofactin produced by Arthrobacter species strain MIS38 was purified and chemically characterized as 3-hydroxydecanoyl-D-leucyl-D-asparagyl-D-threonyl-D- leucyl-D-leucyl-D-seryl-L-leucyl-D-seryl-L-isoleucyl-L-isoleucyl-L-as paragyl lactone. Surface activity of arthrofactin was examined, with surfactin as a control. Critical micelle concentration values of arthrofactin and surfactin were around 1.0 x 10(-5) M and 7.0 x 10(-5) M at 25 degrees C, respectively. Arthrofactin was found to be five to seven times more effective than surfactin. The minimum surface tension value of arthrofactin was 24 mN/m at a concentration higher than the critical micelle concentration. According to the oil displacement assay, arthrofactin was a better oil remover than synthetic surfactants, such as Triton X-100 and sodium dodecyl sulfate. Arthrofactin is one of the most effective lipopeptide biosurfactants. Topics: Amino Acid Sequence; Arthrobacter; Bacterial Proteins; Lipopeptides; Lipoproteins; Magnetic Resonance Spectroscopy; Mass Spectrometry; Molecular Sequence Data; Oligopeptides; Peptides, Cyclic; Solubility; Spectrophotometry, Infrared; Structure-Activity Relationship; Surface-Active Agents | 1993 |