arcyriaflavin-a and rebeccamycin

arcyriaflavin-a has been researched along with rebeccamycin* in 2 studies

Other Studies

2 other study(ies) available for arcyriaflavin-a and rebeccamycin

Article	Year
Characterization and functional modification of StaC and RebC, which are involved in the pyrrole oxidation of indolocarbazole biosynthesis. Bioscience, biotechnology, and biochemistry, 2011, Volume: 75, Issue:11 The diversity of indolocarbazole natural products results from the differences in oxidation states of the pyrroline ring moiety. In the biosynthetic pathways for staurosporine and rebeccamycin, two homologous enzymes having 64% identity, StaC and RebC, are responsible for the selective production of K252c, which has one oxo group at the pyrroline ring, and arcyriaflavin A, which has two. Although StaC has a FAD-binding motif, most StaC molecules do not contain FAD, and the protein cannot be reconstituted with FAD in vitro. In this study, we mutated Ala-118 in StaC by replacing a glutamine that is conserved in FAD monooxygenases, resulting in increased FAD content as well as catalytic activity. In addition, mutations around the substrate-binding sites of StaC and RebC can change the product selectivity. Specifically, StaC-N244R-V246T and RebC-F216V-R239N mutants produced substantial amounts of arcyriaflavin A and K252c, respectively. Topics: Amino Acid Sequence; Binding Sites; Carbazoles; Cloning, Molecular; Flavin-Adenine Dinucleotide; Indole Alkaloids; Indoles; Molecular Sequence Data; Molecular Structure; Oxidation-Reduction; Oxygenases; Pyrroles; Staurosporine; Streptomyces; Substrate Specificity	2011
Generation of potent and selective kinase inhibitors by combinatorial biosynthesis of glycosylated indolocarbazoles. Chemical communications (Cambridge, England), 2009, Jul-21, Issue:27 We report the generation of novel glycosylated indolocarbazoles by combinatorial biosynthesis, and the identification of two novel potent and selective compounds inhibitors of JAK2 and Ikkb kinases. Topics: Antineoplastic Agents; Carbazoles; Cell Line, Tumor; Cell Proliferation; Combinatorial Chemistry Techniques; Humans; Indole Alkaloids; Molecular Structure; Multigene Family; Plasmids; Protein Kinase Inhibitors; Staurosporine; Streptomyces	2009

Article

Year

Characterization and functional modification of StaC and RebC, which are involved in the pyrrole oxidation of indolocarbazole biosynthesis.

Bioscience, biotechnology, and biochemistry, 2011, Volume: 75, Issue:11

The diversity of indolocarbazole natural products results from the differences in oxidation states of the pyrroline ring moiety. In the biosynthetic pathways for staurosporine and rebeccamycin, two homologous enzymes having 64% identity, StaC and RebC, are responsible for the selective production of K252c, which has one oxo group at the pyrroline ring, and arcyriaflavin A, which has two. Although StaC has a FAD-binding motif, most StaC molecules do not contain FAD, and the protein cannot be reconstituted with FAD in vitro. In this study, we mutated Ala-118 in StaC by replacing a glutamine that is conserved in FAD monooxygenases, resulting in increased FAD content as well as catalytic activity. In addition, mutations around the substrate-binding sites of StaC and RebC can change the product selectivity. Specifically, StaC-N244R-V246T and RebC-F216V-R239N mutants produced substantial amounts of arcyriaflavin A and K252c, respectively.

Topics: Amino Acid Sequence; Binding Sites; Carbazoles; Cloning, Molecular; Flavin-Adenine Dinucleotide; Indole Alkaloids; Indoles; Molecular Sequence Data; Molecular Structure; Oxidation-Reduction; Oxygenases; Pyrroles; Staurosporine; Streptomyces; Substrate Specificity

2011

Generation of potent and selective kinase inhibitors by combinatorial biosynthesis of glycosylated indolocarbazoles.

Chemical communications (Cambridge, England), 2009, Jul-21, Issue:27

We report the generation of novel glycosylated indolocarbazoles by combinatorial biosynthesis, and the identification of two novel potent and selective compounds inhibitors of JAK2 and Ikkb kinases.

Topics: Antineoplastic Agents; Carbazoles; Cell Line, Tumor; Cell Proliferation; Combinatorial Chemistry Techniques; Humans; Indole Alkaloids; Molecular Structure; Multigene Family; Plasmids; Protein Kinase Inhibitors; Staurosporine; Streptomyces

2009