arbutin and bergenin

Topics: Adjuvants, Immunologic; Animals; Anti-Inflammatory Agents, Non-Steroidal; Antitussive Agents; Arbutin; Benzopyrans; Conservation of Natural Resources; Drugs, Chinese Herbal; Flavonoids; Humans; Plants, Medicinal; Saxifragaceae

This report describes the fabrication and the application of a novel carbon nanotube (CNT)-epoxy composite electrode as a sensitive amperometric detector for the capillary electrophoresis (CE). The composite electrode was fabricated on the basis of the in situ polycondensation of a mixture of CNTs and 1,2-ethanediamine-containing bisphenol A epoxy resin in the inner bore of a piece of fused silica capillary under heat. It was coupled with CE for the separation and detection of arbutin and bergenin in Bergeniae Rhizoma, a traditional Chinese medicine, to demonstrate its feasibility and performance. The two phenolic constituents were well separated within 10min in a 45cm capillary length at a separation voltage of 12kV using a 50mM borate buffer (pH 9.2). The CNT-based detector offered higher sensitivity, significantly lower operating potential, satisfactory resistance to surface fouling, and lower expense of operation, indicating great promise for a wide range of analytical applications. It showed long-term stability and reproducibility with relative standard deviations of less than 5% for the peak current (n=15).

Topics: Arbutin; Benzhydryl Compounds; Benzopyrans; Buffers; Electrodes; Electrophoresis, Capillary; Epoxy Resins; Equipment Design; Ethylenediamines; Feasibility Studies; Hydrogen-Ion Concentration; Limit of Detection; Linear Models; Nanotubes, Carbon; Phenols; Plant Extracts; Plant Roots; Reproducibility of Results; Saxifragaceae; Surface Properties

To develop a HPLC method for the determination of arbutin, bergenin and catechin in Chinese herb Bergenia, and to provide a scientific basis for evaluating the quality and reasonable utilization of the herb.. The HPLC analysis was achieved by using a C18 column and methanol-water as the mobile phase, with a flow rate of 1.0 mL min(-1), and detected by UV at 275 nm. The contents of arbutin, bergenin and catechin in the different parts of axial root, fibrous root and blade from Bergenia purpurascens and B. crassifolia.. The contents of arbutin, bergenin and catechin have a few difference in B. purpurascens and B. crassifolia, and varies significantly in the different part of axial root, fibrous root and blade from some species. The contents of bergenin are higher in axial root, fibrous root, and the content of arbutin is higher in blade.. This HPLC method can be used to determine simultaneously the content of arbutin, bergenin and catechin, and can establish a foundation for scientific study and evaluating the quality of species in Bergenia.

Topics: Arbutin; Benzopyrans; Catechin; Chromatography, High Pressure Liquid; Plant Components, Aerial; Plant Roots; Reproducibility of Results; Saxifragaceae