araban has been researched along with ferulic-acid* in 7 studies
7 other study(ies) available for araban and ferulic-acid
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Non-covalent interaction between ferulic acid and arabinan-rich pectic polysaccharide from rapeseed meal.
The sorption capacity of arabinan-rich pectic polysaccharide (ARPP) onto ferulic acid (FA) was investigated using equilibrium dialysis assays. UV and FT-IR spectra showed that FA was successfully adsorbed by ARPP. The effects of temperature, pH, buffer concentration, NaCl, and ethanol on sorption were investigated. Sorption variable optimization was examined by response surface methodology. The order of influence of each factor in affecting the sorption capacity was temperature>pH>buffer concentration. The maximum sorption yield was 363.92±18.37μg/mg at 36.8°C, pH 5.26, and a buffer concentration of 0.09M. Langmuir, Freundlich, and Temkin models were used to fit the experimental data under the optimized conditions. The Freundlich model showed the closest fit with an R Topics: Adsorption; Brassica rapa; Coumaric Acids; Ethanol; Hydrogen-Ion Concentration; Osmolar Concentration; Pectins; Polysaccharides; Sodium Chloride; Temperature | 2017 |
Characterization of diferuloylated pectic polysaccharides from quinoa (Chenopodium quinoa WILLD.).
In plants belonging to the order of Caryophyllales, pectic neutral side chains can be substituted with ferulic acid. The ability of ferulic acid to form intra- and/or intermolecular polysaccharide cross-links by dimerization was shown by the isolation and characterization of diferulic acid oligosaccharides from monocotyledonous plants. In this study, two diferulic acid oligosaccharides were isolated from the enzymatic hydrolyzate of seeds of the dicotyledonous pseudocereal quinoa by gel permeation chromatography and preparative HPLC and unambiguously identified by LC-MS(2) and 1D/2D NMR spectroscopy. The isolated oligosaccharides are comprised of 5-5- and 8-O-4-diferulic acid linked to the O2-position of the nonreducing residue of two (1→5)-linked arabinobioses. To get insight into the structure and the degree of phenolic acid substitution of the diferuloylated polysaccharides, polymeric sugar composition, glycosidic linkages, and polysaccharide-bound monomeric phenolic acids and diferulic acids were analyzed. This study demonstrates that diferulic acids are involved into intramolecular and/or intermolecular cross-linking of arabinan chains and may have a major impact on cell wall architecture of quinoa and other dicotyledonous plants of the order of Caryophyllales. Topics: Bolivia; Cell Wall; Chenopodium quinoa; Cinnamates; Coumaric Acids; Nuclear Magnetic Resonance, Biomolecular; Pectins; Polysaccharides; Seeds | 2015 |
Kinetics of enzyme-catalyzed cross-linking of feruloylated arabinan from sugar beet.
Ferulic acid (FA) groups esterified to the arabinan side chains of pectic polysaccharides can be oxidatively cross-linked in vitro by horseradish peroxidase (HRP) catalysis in the presence of hydrogen peroxide (H(2)O(2)) to form ferulic acid dehydrodimers (diFAs). The present work investigated whether the kinetics of HRP catalyzed cross-linking of FA esterified to α-(1,5)-linked arabinans are affected by the length of the arabinan chains carrying the feruloyl substitutions. The kinetics of the HRP-catalyzed cross-linking of four sets of arabinan samples from sugar beet pulp, having different molecular weights and hence different degrees of polymerization, were monitored by the disappearance of FA absorbance at 316 nm. MALDI-TOF/TOF-MS analysis confirmed that the sugar beet arabinans were feruloyl-substituted, and HPLC analysis verified that the amounts of diFAs increased when FA levels decreased as a result of the enzymatic oxidation treatment with HRP and H(2)O(2). At equimolar levels of FA (0.0025-0.05 mM) in the arabinan samples, the initial rates of the HRP-catalyzed cross-linking of the longer chain arabinans were slower than those of the shorter chain arabinans. The lower initial rates may be the result of the slower movement of larger molecules coupled with steric phenomena, making the required initial reaction of two FAs on longer chain arabinans slower than on shorter arabinans. Topics: Beta vulgaris; Biocatalysis; Carbohydrate Sequence; Coumaric Acids; Hydrogen Peroxide; Kinetics; Molecular Sequence Data; Molecular Structure; Molecular Weight; Plant Extracts; Polymerization; Polysaccharides | 2011 |
Sugar beet (Beta vulgaris) pectins are covalently cross-linked through diferulic bridges in the cell wall.
Arabinan and galactan side chains of sugar beet pectins are esterified by ferulic acid residues that can undergo in vivo oxidative reactions to form dehydrodiferulates. After acid and enzymatic degradation of sugar beet cell walls and fractionation of the solubilized products by hydrophobic interaction chromatography, three dehydrodiferulate-rich fractions were isolated. The structural identification of the different compounds present in these fractions was performed by electrospray-ion trap-mass spectrometry (before and after (18)O labeling) and high-performance anion-exchange chromatography. Several compounds contained solely Ara (terminal or alpha-1-->5-linked-dimer) and dehydrodiferulate. The location of the dehydrodiferulate was assigned in some cases to the O-2 and in others to the O-5 of non-reducing Ara residues. One compound contained Gal (beta-1-->4-linked-dimer), Ara (alpha-1-->5-linked-dimer) and dehydrodiferulate. The location of the dehydrodiferulate was unambiguously assigned to the O-2 of the non-reducing Ara residue and O-6 of the non-reducing Gal residue. These results provide direct evidence that pectic arabinans and galactans are covalently cross-linked (intra- or inter-molecularly) through dehydrodiferulates in sugar beet cell walls. Molecular modeling was used to compute and structurally characterize the low energy conformations of the isolated compounds. Interestingly, the conformations of the dehydrodiferulate-bridged arabinan and galactan fragments selected from an energetic criterion, evidenced very nice agreement with the experimental occurrence of the dehydrodiferulated pectins. The present work combines for the first time intensive mass spectrometry data and molecular modeling to give structural relevance of a molecular cohesion between rhamnogalacturonan fragments. Topics: Beta vulgaris; Carbohydrate Conformation; Carbohydrate Sequence; Cell Wall; Coumaric Acids; Galactans; Mass Spectrometry; Models, Molecular; Molecular Sequence Data; Pectins; Polysaccharides | 2005 |
Isolation from sugar beet cell walls of arabinan oligosaccharides esterified by two ferulic acid monomers.
Side chains of sugar beet (Beta vulgaris) pectins, which are mainly composed of arabinose (Ara) and galactose (Gal) residues, are esterified by ferulic acid units. Enzymatic hydrolysis of beet cell walls yielded several feruloylated oligosaccharides, which were separated by hydrophobic interaction chromatography. Two new oligomers were isolated in the fraction eluted by 25:75 (v/v) ethanol:water. An arabinotriose and an arabinotetraose esterified by two ferulic acid residues were obtained, and their structure was elucidated by mass spectrometry. It is shown that feruloyl groups are linked to O-5 of Ara residues, in addition to the known O-2 position. This work establishes for the first time, to our knowledge, that two neighboring Ara units may be esterified by two ferulic acid units. This close proximity may have important biochemical implications. Topics: Beta vulgaris; Cell Wall; Coumaric Acids; Esterification; Molecular Structure; Oligosaccharides; Polysaccharides; Spectrometry, Mass, Electrospray Ionization | 2004 |
Isolation of diferulic bridges ester-linked to arabinan in sugar beet cell walls.
After degradation of sugar beet cell walls with Driselase and fractionation of the solubilised products by hydrophobic interaction chromatography, a dehydrodiferuloylated oligoarabinan was isolated. Its structure was assigned to two dimers of (1-->5)-linked arabinose units esterified by a central 8-O-4' ferulic dimer. These results provide the first direct evidence that pectic arabinans in sugar beet cell walls may be covalently cross-linked through dehydrodiferulates. Topics: Beta vulgaris; Chromatography, Gel; Coumaric Acids; Dimerization; Indicators and Reagents; Mass Spectrometry; Oligosaccharides; Polysaccharides; Spectrometry, Mass, Electrospray Ionization | 2004 |
Oxidative cross-linking of pectic polysaccharides from sugar beet pulp.
Oxidative cross-linking of three beet pectin extracts with hydrogen peroxide/peroxidase resulted in an increase in viscosity at low concentrations and in the formation of a gel at higher concentrations. Gels were formed using concentrations of 1.5% for an autoclave preparation and one obtained by an acid extraction and of 3% for a second autoclaved extract. It was shown that in the autoclave extracts only rhamnogalacturonans and possibly the arabinans participated in the cross-linking reaction. Cross-linking of the autoclave extracts with ammonium persulfate resulted in a decrease in reduced viscosity and molecular weight, although ferulic acid dehydrodimers were formed. Treatment of the acid extracted pectin with ammonium persulfate gave a slow increase in viscosity and the formation of a high-molecular-weight population was observed. For both oxidative systems, the 8-5 dehydrodimer was predominant after cross-linking. Topics: Ammonium Sulfate; Chenopodiaceae; Chromatography, Gel; Coumaric Acids; Cross-Linking Reagents; Dimerization; Food Additives; Gels; Hot Temperature; Hydrogen Peroxide; Molecular Weight; Oxidation-Reduction; Pectins; Peroxidase; Polysaccharides; Viscosity | 2000 |