apyrase and purine

apyrase has been researched along with purine* in 2 studies

Other Studies

2 other study(ies) available for apyrase and purine

ArticleYear
Anti-Inflammatory Effects of Quercetin on High-Glucose and Pro-Inflammatory Cytokine Challenged Vascular Endothelial Cell Metabolism.
    Molecular nutrition & food research, 2021, Volume: 65, Issue:6

    Pro-inflammatory stimuli such as hyperglycemia and cytokines have been shown to negatively affect endothelial cell functions. The aim of this study is to assess the potential of quercetin and its human metabolites to overcome the deleterious effects of hyperglycemic or inflammatory conditions on the vascular endothelium by modulating endothelial cell metabolism.. A metabolomics approach enabled identification and quantification of 27 human umbilical vein endothelial cell (HUVEC) metabolites. Treatment of HUVECs with high-glucose concentrations causes significant increases in lactate and glutamate concentrations. Quercetin inhibits glucose-induced increases in lactate and adenosine 5'-triphosphate (ATP) and also increased inosine concentrations. Tumor necrosis factor α-treatment (TNFα) of HUVECs causes increases in asparagine and decreases in aspartate concentrations. Co-treatment with quercetin reduces pyruvate concentrations compared to TNFα-only treated controls. Subsequently, it was shown that quercetin and its HUVEC phase-2 conjugates inhibit adenosine deaminase, xanthine oxidase and 5'nucleotidase (CD73) but not ectonucleoside triphosphate diphosphohydrolase-1 (CD39) or purine nucleoside phosphorylase activities.. Quercetin was shown to alter the balance of HUVEC metabolites towards a less inflamed phenotype, both alone and in the presence of pro-inflammatory stimuli. These changes are consistent with the inhibition of particular enzymes involved in purine metabolism by quercetin and its HUVEC metabolites.

    Topics: 5'-Nucleotidase; Adenosine Deaminase; Apyrase; Cytokines; Endothelium, Vascular; Energy Metabolism; Glucose; GPI-Linked Proteins; Human Umbilical Vein Endothelial Cells; Humans; Inflammation; Purine-Nucleoside Phosphorylase; Purines; Quercetin; Tumor Necrosis Factor-alpha; Xanthine Oxidase

2021
Induction of antiinflammatory purinergic signaling in activated human iNKT cells.
    JCI insight, 2018, 09-06, Volume: 3, Issue:17

    Invariant natural killer T (iNKT) cells are activated at sites of local tissue injury, or globally during vaso-occlusive episodes of sickle cell disease (SCD). Tissue damage stimulates production of CD1d-restricted lipid antigens that activate iNKT cells to produce Th1- and Th2-type cytokines. Here, we show that circulating iNKT cells in SCD patients express elevated levels of the ectonucleoside triphosphate diphosphosphohydrolase, CD39, as well the adenosine A2A receptor (A2AR). We also investigated the effects of stimulating cultured human iNKT cells on the expression of genes involved in the regulation of purinergic signaling. iNKT cell stimulation caused induction of ADORA2A, P2RX7, CD38, CD39, ENPP1, CD73, PANX1, and ENT1. Transcription of ADA, which degrades adenosine, was reduced. Induction of CD39 mRNA was associated with increased ecto-ATPase activity on iNKT cells that was blocked by POM1. Exposure of iNKT cells to A2AR agonists during stimulation reduced production of IFN-γ and enhanced production of IL-13 and CD39. Based on these findings, we define "purinergic Th2-type cytokine bias" as an antiinflammatory purinergic response to iNKT cell stimulation resulting from changes in the transcription of several genes involved in purine release, extracellular metabolism, and signaling.

    Topics: 5'-Nucleotidase; ADP-ribosyl Cyclase 1; Anemia, Sickle Cell; Antigens, CD1d; Apyrase; Connexins; Cytokines; Equilibrative Nucleoside Transporter 1; GPI-Linked Proteins; Humans; Immunity, Innate; Interleukin-13; Natural Killer T-Cells; Nerve Tissue Proteins; Phosphoric Diester Hydrolases; Purinergic Agents; Purines; Pyrophosphatases; Receptor, Adenosine A2A; Receptors, Purinergic P2X7; Signal Transduction; Transcription Factors

2018