apyrase and cerivastatin

apyrase has been researched along with cerivastatin* in 1 studies

Other Studies

1 other study(ies) available for apyrase and cerivastatin

ArticleYear
Reversal of thrombin-induced deactivation of CD39/ATPDase in endothelial cells by HMG-CoA reductase inhibition: effects on Rho-GTPase and adenosine nucleotide metabolism.
    Arteriosclerosis, thrombosis, and vascular biology, 2002, Jun-01, Volume: 22, Issue:6

    Adenosine triphosphate and diphosphate that activate platelet, leukocyte, and endothelium functions are hydrolyzed by endothelial CD39/ATPDase. Because CD39/ATPDase is downregulated in endothelial cells by inflammation and this may be affected by HMG-CoA reductase inhibitors, we examined the role of cerivastatin and simvastatin in regulation of endothelial CD39/ATPDase expression, metabolism of ATP/ADP, and function in platelets. Thrombin-stimulated endothelial cells in vitro were treated with the statins, and hydrolysis of exogenous ADP and ATP was assessed by high-performance liquid chromatography and malachite green assay. Platelet aggregation studies were performed with endothelial cell supernatants as triggers. CD39/ATPDase surface expression by endothelial cells was determined immunologically by fluorescence-activated cell sorter, mRNA expression by RT-PCR, and thrombin-induced dissociation of Rho-GTPases by Western blotting. Treatment by simvastatin or cerivastatin restored impaired metabolism of exogenous ATP and ADP in thrombin-activated endothelial cells by preventing thrombin-induced downregulation of CD39/ATPDase. In platelet aggregation studies, ATP and ADP supernatants of thrombin-activated endothelial cells were less stimulatory in the presence of statins than in their absence. Data show that statins preserve CD39/ATPDase activity in thrombin-treated endothelial cells involving alterations by statins of Rho-GTPase function and CD39/ATPDase expression. Preservation of adenine nucleotide metabolism may directly contribute to the observed anti-thrombotic and anti-inflammatory actions of statins.

    Topics: Adenosine Diphosphate; Adenosine Triphosphatases; Adenosine Triphosphate; Antigens, CD; Apyrase; Blood Platelets; Cell Separation; Cells, Cultured; Culture Media, Conditioned; Endothelium, Vascular; Enzyme Reactivators; Female; Humans; Hydroxymethylglutaryl-CoA Reductase Inhibitors; Kinetics; Platelet Aggregation; Pyridines; rho GTP-Binding Proteins; Simvastatin; Thrombin; Time Factors; Umbilical Veins

2002