aphidicolin and acetylleucyl-leucyl-norleucinal

aphidicolin has been researched along with acetylleucyl-leucyl-norleucinal* in 2 studies

Other Studies

2 other study(ies) available for aphidicolin and acetylleucyl-leucyl-norleucinal

Article	Year
Cell cycle analysis and synchronization of the Xenopus cell line XL2. Experimental cell research, 1998, Jul-10, Volume: 242, Issue:1 We have determined the length of the cell cycle and its different phases in a permanent Xenopus tadpole cell line, XL2. Following BrdU labeling, the total length of the cell cycle was estimated as 28 h. The different phases of the cell cycle, G1, S, G2, and M were, respectively, 14 h, 10 h 45 min, 2 h 30 min, and 54 min. Knowing these parameters, we were able to develop methods that selectively enrich cells in different phases of the cycle. Treatment with aphidicolin resulted in a S phase block in which more than 85% of the cells showed S phase chromosomes. Almost 60% of the cells were arrested in mitosis after a double block with aphidicolin/nocodazole or aphidicolin/ALLN (acetyl-leucyl-leucyl-norleucinal) treatment. This synchronization protocol will greatly facilitate studies of biochemical events associated with specific gene regulation through the cell cycle. Our synchronization protocol does not disturb cell metabolism as the expression of cyclin B2 during the cell cycle is in agreement with the results obtained with mammalian cells. Topics: Animals; Aphidicolin; Cell Cycle; Cell Line; Cyclin B; Leupeptins; Mitosis; Nocodazole; S Phase; Tubulin; Xenopus laevis	1998
Coupling of mitosis to the completion of S phase through Cdc34-mediated degradation of Wee1. Science (New York, N.Y.), 1998, Dec-04, Volume: 282, Issue:5395 The dependence of mitosis on the completion of the period of DNA replication in the cell cycle [synthesis (S) phase] ensures that chromosome segregation occurs only after the genome has been fully duplicated. A key negative regulator of mitosis, the protein kinase Wee1, was degraded in a Cdc34-dependent fashion in Xenopus egg extracts. This proteolysis event was required for a timely entrance into mitosis and was inhibited when DNA replication was blocked. Therefore, the DNA replication checkpoint can prevent mitosis by suppressing the proteolysis of Wee1 during S phase. Topics: Anaphase-Promoting Complex-Cyclosome; Animals; Aphidicolin; cdc25 Phosphatases; Cell Cycle Proteins; Cell Nucleus; Cyclin-Dependent Kinase Inhibitor p27; DNA Replication; Female; G2 Phase; Leupeptins; Ligases; Male; Maturation-Promoting Factor; Microtubule-Associated Proteins; Mitosis; Nuclear Proteins; Okadaic Acid; Ovum; Phosphoprotein Phosphatases; Protein Serine-Threonine Kinases; Protein-Tyrosine Kinases; Proteins; Recombinant Proteins; S Phase; Spermatozoa; Tumor Suppressor Proteins; Ubiquitin-Protein Ligase Complexes; Ubiquitin-Protein Ligases; Ubiquitins; Xenopus; Xenopus Proteins	1998

Article

Year

Cell cycle analysis and synchronization of the Xenopus cell line XL2.

Experimental cell research, 1998, Jul-10, Volume: 242, Issue:1

We have determined the length of the cell cycle and its different phases in a permanent Xenopus tadpole cell line, XL2. Following BrdU labeling, the total length of the cell cycle was estimated as 28 h. The different phases of the cell cycle, G1, S, G2, and M were, respectively, 14 h, 10 h 45 min, 2 h 30 min, and 54 min. Knowing these parameters, we were able to develop methods that selectively enrich cells in different phases of the cycle. Treatment with aphidicolin resulted in a S phase block in which more than 85% of the cells showed S phase chromosomes. Almost 60% of the cells were arrested in mitosis after a double block with aphidicolin/nocodazole or aphidicolin/ALLN (acetyl-leucyl-leucyl-norleucinal) treatment. This synchronization protocol will greatly facilitate studies of biochemical events associated with specific gene regulation through the cell cycle. Our synchronization protocol does not disturb cell metabolism as the expression of cyclin B2 during the cell cycle is in agreement with the results obtained with mammalian cells.

Topics: Animals; Aphidicolin; Cell Cycle; Cell Line; Cyclin B; Leupeptins; Mitosis; Nocodazole; S Phase; Tubulin; Xenopus laevis

1998

Coupling of mitosis to the completion of S phase through Cdc34-mediated degradation of Wee1.

Science (New York, N.Y.), 1998, Dec-04, Volume: 282, Issue:5395

The dependence of mitosis on the completion of the period of DNA replication in the cell cycle [synthesis (S) phase] ensures that chromosome segregation occurs only after the genome has been fully duplicated. A key negative regulator of mitosis, the protein kinase Wee1, was degraded in a Cdc34-dependent fashion in Xenopus egg extracts. This proteolysis event was required for a timely entrance into mitosis and was inhibited when DNA replication was blocked. Therefore, the DNA replication checkpoint can prevent mitosis by suppressing the proteolysis of Wee1 during S phase.

Topics: Anaphase-Promoting Complex-Cyclosome; Animals; Aphidicolin; cdc25 Phosphatases; Cell Cycle Proteins; Cell Nucleus; Cyclin-Dependent Kinase Inhibitor p27; DNA Replication; Female; G2 Phase; Leupeptins; Ligases; Male; Maturation-Promoting Factor; Microtubule-Associated Proteins; Mitosis; Nuclear Proteins; Okadaic Acid; Ovum; Phosphoprotein Phosphatases; Protein Serine-Threonine Kinases; Protein-Tyrosine Kinases; Proteins; Recombinant Proteins; S Phase; Spermatozoa; Tumor Suppressor Proteins; Ubiquitin-Protein Ligase Complexes; Ubiquitin-Protein Ligases; Ubiquitins; Xenopus; Xenopus Proteins

1998