anticodon and 5-6-dihydrouridine

anticodon has been researched along with 5-6-dihydrouridine* in 2 studies

Other Studies

2 other study(ies) available for anticodon and 5-6-dihydrouridine

Article	Year
Assignment of the magnetic resonances of the imino protons and methyl protons of Bombyx mori tRNA(GlyGCC) and the effect of ion binding on its structure. European journal of biochemistry, 1992, Dec-15, Volume: 210, Issue:3 The magnetic resonances in the low-field H-NMR spectra of Bombyx mori tRNA(GlyGCC), corresponding to the hydrogen-bonded imino protons of the helical stems and tertiary base pairs, could be tentatively assigned by means of the sequential nuclear Overhauser effects. While B. mori tRNA(GlyGCC) does not contain the G19C56 tertiary base pair, the D20G57 base pair exists between the D and T loops, which was not found in the X-ray crystal structure of yeast tRNA(Phe). The effects of Mg2+, spermine and temperature on the conformation of this tRNA have also been examined based on the behavior of the assigned resonance signals. Mg2+ stabilize the D and T stems and the tertiary structure between the D and T loops. Spermine affects the resonances of the D and anticodon stems, and A23G9, but does not stabilize them. While the acceptor stem melts sequentially from both ends (G7C66 and G1C72) with increasing temperature, the anticodon stem melts from only one end (G39C31) and the G26C44 base pair is the most stable. In the tertiary structure between the variable loop and D stem, G10G45 melts first and G22G46 last. Yeast tRNA(Phe) has also been examined, and the results were compared with those for B. mori tRNA(Gly). Topics: Animals; Anticodon; Base Composition; Base Sequence; Bombyx; Codon; Hydrogen Bonding; Magnesium; Magnetic Resonance Spectroscopy; Molecular Sequence Data; Nucleic Acid Conformation; RNA, Transfer, Gly; RNA, Transfer, Phe; Saccharomyces cerevisiae; Spermine; Uridine	1992
A mammalian mitochondrial serine transfer RNA lacking the "dihydrouridine" loop and stem. Nucleic acids research, 1980, Nov-25, Volume: 8, Issue:22 A unique transfer RNA has been identified in human and bovine mitochondria that lacks the "dihydrouridine" loop and stem structure. This tRNA is mitochondrially coded as shown by DNA sequence analysis of the human and bovine mitochondrial DNA. Sequence analysis of the RNA shows that it is post-transcriptionally modified by the addition of CCA at the 3' terminus and that at least one base is modified. As predicted by its anticodon (GCU, corresponding to the serine codons AGU/C) this tRNA can be aminoacylated with serine when purified mitochondria are incubated in a medium containing 3H-serine. Topics: Animals; Anticodon; Base Sequence; Cattle; Cloning, Molecular; DNA, Recombinant; Humans; Mitochondria, Liver; Nucleic Acid Conformation; Plasmids; RNA, Transfer, Amino Acyl; Species Specificity; Uridine	1980

Article

Year

Assignment of the magnetic resonances of the imino protons and methyl protons of Bombyx mori tRNA(GlyGCC) and the effect of ion binding on its structure.

European journal of biochemistry, 1992, Dec-15, Volume: 210, Issue:3

The magnetic resonances in the low-field H-NMR spectra of Bombyx mori tRNA(GlyGCC), corresponding to the hydrogen-bonded imino protons of the helical stems and tertiary base pairs, could be tentatively assigned by means of the sequential nuclear Overhauser effects. While B. mori tRNA(GlyGCC) does not contain the G19C56 tertiary base pair, the D20G57 base pair exists between the D and T loops, which was not found in the X-ray crystal structure of yeast tRNA(Phe). The effects of Mg2+, spermine and temperature on the conformation of this tRNA have also been examined based on the behavior of the assigned resonance signals. Mg2+ stabilize the D and T stems and the tertiary structure between the D and T loops. Spermine affects the resonances of the D and anticodon stems, and A23G9, but does not stabilize them. While the acceptor stem melts sequentially from both ends (G7C66 and G1C72) with increasing temperature, the anticodon stem melts from only one end (G39C31) and the G26C44 base pair is the most stable. In the tertiary structure between the variable loop and D stem, G10G45 melts first and G22G46 last. Yeast tRNA(Phe) has also been examined, and the results were compared with those for B. mori tRNA(Gly).

Topics: Animals; Anticodon; Base Composition; Base Sequence; Bombyx; Codon; Hydrogen Bonding; Magnesium; Magnetic Resonance Spectroscopy; Molecular Sequence Data; Nucleic Acid Conformation; RNA, Transfer, Gly; RNA, Transfer, Phe; Saccharomyces cerevisiae; Spermine; Uridine

1992

A mammalian mitochondrial serine transfer RNA lacking the "dihydrouridine" loop and stem.

Nucleic acids research, 1980, Nov-25, Volume: 8, Issue:22

A unique transfer RNA has been identified in human and bovine mitochondria that lacks the "dihydrouridine" loop and stem structure. This tRNA is mitochondrially coded as shown by DNA sequence analysis of the human and bovine mitochondrial DNA. Sequence analysis of the RNA shows that it is post-transcriptionally modified by the addition of CCA at the 3' terminus and that at least one base is modified. As predicted by its anticodon (GCU, corresponding to the serine codons AGU/C) this tRNA can be aminoacylated with serine when purified mitochondria are incubated in a medium containing 3H-serine.

Topics: Animals; Anticodon; Base Sequence; Cattle; Cloning, Molecular; DNA, Recombinant; Humans; Mitochondria, Liver; Nucleic Acid Conformation; Plasmids; RNA, Transfer, Amino Acyl; Species Specificity; Uridine

1980